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Literature summary extracted from

  • Yao, P.; Cui, Y.; Yu, S.; Du, Y.; Feng, J.; Wu, Q.; Zhu, D.
    Efficient biosynthesis of (R)- or (S)-2-hydroxybutyrate from L-threonine through a synthetic biology approach (2016), Adv. Synth. Catal., 358, 2923-2928.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.28 expressed in Escherichia coli BL21(DE3) cells Staphylococcus epidermidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 2-oxobutyrate substrate inhibition Staphylococcus epidermidis
1.1.1.28 additional information L-threonine and 2-hydroxybutyrate do not affect enzyme activity up to 200 mM Staphylococcus epidermidis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 pyruvate + NADH + H+ Staphylococcus epidermidis
-
 (R)-lactate + NAD+
-
 ?
1.1.1.28 pyruvate + NADH + H+ Staphylococcus epidermidis ATCC 12228
-
 (R)-lactate + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Staphylococcus epidermidis
-
-
-
1.1.1.28 Staphylococcus epidermidis ATCC 12228
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 pyruvate + NADH + H+
-
 Staphylococcus epidermidis (R)-lactate + NAD+
-
 ?
1.1.1.28 pyruvate + NADH + H+
-
 Staphylococcus epidermidis ATCC 12228 (R)-lactate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 D-lactate dehydrogenase
-
 Staphylococcus epidermidis
1.1.1.28 D-LDH
-
 Staphylococcus epidermidis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NADH
-
 Staphylococcus epidermidis