EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.169 | gene TK1968, recombinant coexpression of wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer in Escherichia coli | Thermococcus kodakarensis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.169 | purified recombinant tagged enzyme mutant C84A, hanging drop vapour diffusion method, mixing of 0.001 ml of 10 mg /ml protein in 10 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, mM NADH, and 1 mM 2-oxopantoate, with 0.001 ml of reservoir solution containing 100 mM sodium acetate, pH 5.5, 10% w/v PEG 3350, 20% v/v 2-propanol, and equilibration against 0.5 ml reservoir solution, at 20°C, 1 week, X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method for modeling | Thermococcus kodakarensis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.169 | C84A | site-directed mutagenesis | Thermococcus kodakarensis |
1.1.1.169 | W129A | site-directed mutagenesis | Thermococcus kodakarensis |
1.1.1.169 | Y60A | site-directed mutagenesis | Thermococcus kodakarensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | CoA | feedback inhibition | Thermococcus kodakarensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | (R)-pantoate + NADP+ | Thermococcus kodakarensis | - |
2-dehydropantoate + NADPH + H+ | - |
r | |
1.1.1.169 | (R)-pantoate + NADP+ | Thermococcus kodakarensis ATCC BAA-918 | - |
2-dehydropantoate + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.169 | Thermococcus kodakarensis | Q5JGC2 | - |
- |
1.1.1.169 | Thermococcus kodakarensis ATCC BAA-918 | Q5JGC2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.169 | recombinant wild-type and mutant Tk-KPR enzyme dimers carrying a His6-tag and a Strep-tag on each monomer from Escherichia coli by affinity chromatography | Thermococcus kodakarensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | (R)-pantoate + NADP+ | - |
Thermococcus kodakarensis | 2-dehydropantoate + NADPH + H+ | - |
r | |
1.1.1.169 | (R)-pantoate + NADP+ | - |
Thermococcus kodakarensis ATCC BAA-918 | 2-dehydropantoate + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.169 | dimer | crystal structure analysis | Thermococcus kodakarensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.169 | ketopantoate reductase | - |
Thermococcus kodakarensis |
1.1.1.169 | KPR | - |
Thermococcus kodakarensis |
1.1.1.169 | Tk-KPR | - |
Thermococcus kodakarensis |
1.1.1.169 | TK1968 | - |
Thermococcus kodakarensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 70 | - |
assay at | Thermococcus kodakarensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 6.4 | - |
assay at | Thermococcus kodakarensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | NADP+ | - |
Thermococcus kodakarensis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.169 | physiological function | ketopantoate reductase catalyzes the NAD(P)H-dependent reduction of 2-oxopantoate which is a step in the biosynthesis of coenzyme A | Thermococcus kodakarensis |