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Literature summary extracted from

  • Kim, J.; Kim, K.
    Cloning, expression, purification, crystallization and X-ray crystallographic analysis of the (S)-3-hydroxybutyryl-CoA dehydrogenase PaaH1 from Ralstonia eutropha H16 (2014), Acta Crystallogr. Sect. F, 70, 955-958.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.35 synthesis the enzyme is used in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to (S)-3-hydroxybutyryl-CoA Cupriavidus necator

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.35 gene paaH1, recombinant expression of C-terminally His6-tagged wild-type or selenomethionine-labeled enzymes in Escherichia coli strain B834 Cupriavidus necator

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.35 purified recombinant wild-type and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, mixing of 30 mg/ml wild-type protein or 25 mg/ml selenomethionine-labeled enzyme in 40 mM Tris-HCl, pH 8.0, 1 mM DTT, with reservoir solution containing 1.4 M ammonium sulfate, 0.1 M sodium cacodylate, pH 6.0, and 0.2 M sodium chloride, 22°C, 7 days, X-ray diffraction structure determination and analysis at 2.6 A resolution, single-wavelength anomalous dispersion method Cupriavidus necator

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+ Cupriavidus necator
-
 acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+ Cupriavidus necator ATCC 17699
-
 acetoacetyl-CoA + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Cupriavidus necator Q0KEY8
-
-
1.1.1.35 Cupriavidus necator ATCC 17699 Q0KEY8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 recombinant C-terminally His6-tagged wild-type or selenomethionine-labeled enzymes from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Cupriavidus necator acetoacetyl-CoA + NADH + H+
-
 r
1.1.1.35 (S)-3-hydroxybutanoyl-CoA + NAD+
-
 Cupriavidus necator ATCC 17699 acetoacetyl-CoA + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.35 (S)-3-hydroxybutyryl-CoA dehydrogenase
-
 Cupriavidus necator
1.1.1.35 PaaH1
-
 Cupriavidus necator

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Cupriavidus necator
1.1.1.35 NADH
-
 Cupriavidus necator

General Information

EC Number General Information Comment Organism
1.1.1.35 metabolism the enzyme catalyzes the second step in the biosynthesis of n-butanol from acetyl-CoA by the reduction of acetoacetyl-CoA to 3-hydroxybutyryl-CoA Cupriavidus necator