Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhang, L.; Chen, J.; Mori, T.; Yan, Y.; Liu, W.; Abe, I.
    Crystallization and preliminary X-ray diffraction analysis of AntE, a crotonyl-CoA carboxylase/reductase from Streptomyces sp. NRRL 2288 (2014), Acta Crystallogr. Sect. F, 70, 734-737.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.85 expressed in Escherichia coli BLR(DE3) cells Streptomyces sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.85 sitting drop vapor diffusion method, using 100 mM CAPS-NaOH pH 10.0, 38% (v/v) PEG 400 Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.85 47000
-
 4 * 47000, SDS-PAGE Streptomyces sp.
1.3.1.85 143000
-
 gel filtration Streptomyces sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+ Streptomyces sp.
-
 (2S)-ethylmalonyl-CoA + NADP+
-
 ?
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+ Streptomyces sp. NRRL 2288
-
 (2S)-ethylmalonyl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.85 Streptomyces sp. M1SQA1
-
-
1.3.1.85 Streptomyces sp. NRRL 2288 M1SQA1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.85 Ni-NTA column chromatography and Superdex 200HR gel filtration Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.85 (2E)-3-cyclohexylprop-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-2-carboxy-3-cyclohexylpropanoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-3-cyclohexylprop-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. NRRL 2288 (2S)-2-carboxy-3-cyclohexylpropanoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-4-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. [(2R)-2-methylbutyl]propanedioyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-4-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. NRRL 2288 [(2R)-2-methylbutyl]propanedioyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-5-chloropent-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-2-carboxy-5-chloropentanoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-5-chloropent-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. NRRL 2288 (2S)-2-carboxy-5-chloropentanoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-5-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-(3-methylbutyl)propanedioyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-5-methylhex-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. NRRL 2288 (2S)-(3-methylbutyl)propanedioyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-6-methyloct-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-2-carboxy-6-methyloctanoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-hept-2-en-6-ynoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-2-craboxy-hept-6-ynoyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-hex-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (S)-2-butylpropanedioyl-CoA + NADP+
-
 ?
1.3.1.85 (2E)-oct-2-enoyl-CoA + NADPH + CO2 + H+
-
 Streptomyces sp. (2S)-2-carboxyoctanoyl-CoA + NADP+
-
 ?
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+
-
 Streptomyces sp. (2S)-ethylmalonyl-CoA + NADP+
-
 ?
1.3.1.85 crotonyl-CoA + CO2 + NADPH + H+
-
 Streptomyces sp. NRRL 2288 (2S)-ethylmalonyl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.85 homotetramer 4 * 47000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.3.1.85 AntE
-
 Streptomyces sp.
1.3.1.85 CCR
-
 Streptomyces sp.
1.3.1.85 crotonyl-CoA carboxylase/reductase
-
 Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.85 NADPH
-
 Streptomyces sp.