Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schwentke, J.; Sabel, A.; Petri, A.; Koenig, H.; Claus, H.
    The yeast Wickerhamomyces anomalus AS1 secretes a multifunctional exo-beta-1,3-glucanase with implications for winemaking (2014), Yeast, 31, 349-359.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.58 food industry Exg2 is active under typical wine-related conditions, such as low pH (3.5–4.0), high sugar concentrations (up to 20% w/v), high ethanol concentrations (10–15% v/v), presence of sulfites (up to 2 mM) and various cations and may have multiple uses in wine making Wickerhamomyces anomalus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 Ca2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus
3.2.1.58 Cu2+ 1 mM, 94% of initial activity Wickerhamomyces anomalus
3.2.1.58 K+ 1 mM, 88% of initial activity Wickerhamomyces anomalus
3.2.1.58 Mg2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus
3.2.1.58 Mn2+ 1 mM, 89% of initial activity Wickerhamomyces anomalus
3.2.1.58 additional information the enzyme is active under typical wine-related conditions, such as low pH (3.5-4.0), high sugar concentrations (up to 20% w/v), high ethanol concentrations (10-15% v/v), presence of sulfites (up to 2 mM) and various cations Wickerhamomyces anomalus
3.2.1.58 potassium metabisulfite 1 mM, 90% of initial activity Wickerhamomyces anomalus
3.2.1.58 Zn2+ 1 mM, 100% of initial activity Wickerhamomyces anomalus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.58 extracellular
-
 Wickerhamomyces anomalus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 47456
-
-
 Wickerhamomyces anomalus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.58 Ethanol 10-15% v/v, about 70% of maximum actviity Wickerhamomyces anomalus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Wickerhamomyces anomalus H6UWP3
-
-
3.2.1.58 Wickerhamomyces anomalus AS1 H6UWP3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 arbutin + H2O
-
 Wickerhamomyces anomalus D-glucose + ?
-
 ?
3.2.1.58 arbutin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + ?
-
 ?
3.2.1.58 cellobiose + H2O
-
 Wickerhamomyces anomalus 2 D-glucose
-
 ?
3.2.1.58 cellobiose + H2O
-
 Wickerhamomyces anomalus AS1 2 D-glucose
-
 ?
3.2.1.58 esculin + H2O
-
 Wickerhamomyces anomalus D-glucose + 4-hydroxyphenol
-
 ?
3.2.1.58 esculin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + 4-hydroxyphenol
-
 ?
3.2.1.58 gentobiose + H2O
-
 Wickerhamomyces anomalus 2 D-glucose
-
 ?
3.2.1.58 gentobiose + H2O
-
 Wickerhamomyces anomalus AS1 2 D-glucose
-
 ?
3.2.1.58 laminarin + H2O
-
 Wickerhamomyces anomalus D-glucose + ? glucose is the sole hydrolysis product ?
3.2.1.58 laminarin + H2O
-
 Wickerhamomyces anomalus AS1 D-glucose + ? glucose is the sole hydrolysis product ?
3.2.1.58 polydatin + H2O
-
 Wickerhamomyces anomalus D-glucose + 3,4',5-trihydroxystilbene
-
 ?
3.2.1.58 salicin + H2O
-
 Wickerhamomyces anomalus D-glucose + 2-hydroxymethylphenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 47456, caluculated, mature protein Wickerhamomyces anomalus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 EXG2
-
 Wickerhamomyces anomalus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 60
-
-
 Wickerhamomyces anomalus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4.5 5
-
 Wickerhamomyces anomalus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.58 Wickerhamomyces anomalus calculated
-
 4.8