Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yoshida, S.; Hiraga, K.; Takehana, T.; Taniguchi, I.; Yamaji, H.; Maeda, Y.; Toyohara, K.; Miyamoto, K.; Kimura, Y.; Oda, K.
    A bacterium that degrades and assimilates poly(ethylene terephthalate) (2016), Science, 351, 1196-1199.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.101 degradation enzyme shows good activity against commercial bottle-derived PET, which is highly crystallized and is was considerably active against PET film at low temperatures Ideonella sakaiensis
3.1.1.102 degradation enzyme shows good activity against commercial bottle-derived PET, which is highly crystallized and is considerably active against PET film at low temperatures Ideonella sakaiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.102 0.0073
-
 ethylene terephthalate pH 7.0, 30°C Ideonella sakaiensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.101 Ideonella sakaiensis A0A0K8P6T7
-
-
3.1.1.101 Ideonella sakaiensis 201-F6 A0A0K8P6T7
-
-
3.1.1.102 Ideonella sakaiensis A0A0K8P8E7
-
-
3.1.1.102 Ideonella sakaiensis 201-F6 A0A0K8P8E7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Ideonella sakaiensis (ethylene terephthalate)n-1 + mono(2-hydroxyethyl)terephthalic acid mono(2-hydroxyethyl)terephthalic acid is the major product, plus minor amounts of terephthalic acid and bis(2-hydroxyethyl) terephthalic acid ?
3.1.1.101 (ethylene terephthalate)n + H2O
-
 Ideonella sakaiensis 201-F6 (ethylene terephthalate)n-1 + mono(2-hydroxyethyl)terephthalic acid mono(2-hydroxyethyl)terephthalic acid is the major product, plus minor amounts of terephthalic acid and bis(2-hydroxyethyl) terephthalic acid ?
3.1.1.101 bis(2-hydroxyethyl) terephthalic acid + H2O
-
 Ideonella sakaiensis mono(2-hydroxyethyl)terephthalic acid + ethylene glycol enzyme does not catalyze further decomposition of mono(2-hydroxyethyl)terephthalic acid ?
3.1.1.101 bis(2-hydroxyethyl) terephthalic acid + H2O
-
 Ideonella sakaiensis 201-F6 mono(2-hydroxyethyl)terephthalic acid + ethylene glycol enzyme does not catalyze further decomposition of mono(2-hydroxyethyl)terephthalic acid ?
3.1.1.101 additional information enzyme shows low activity on 4-nitrophenyl aliphatic esters Ideonella sakaiensis ?
-
 ?
3.1.1.101 additional information enzyme shows low activity on 4-nitrophenyl aliphatic esters Ideonella sakaiensis 201-F6 ?
-
 ?
3.1.1.102 ethylene terephthalate + H2O
-
 Ideonella sakaiensis terephthalate + ethylene glycol
-
 ?
3.1.1.102 ethylene terephthalate + H2O
-
 Ideonella sakaiensis 201-F6 terephthalate + ethylene glycol
-
 ?
3.1.1.102 additional information no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters Ideonella sakaiensis ?
-
 ?
3.1.1.102 additional information no substrates: poly(ethylene terephthalate), bis(2-hydroxyethyl) terephthalic acid or 4-nitrophenyl aliphatic esters Ideonella sakaiensis 201-F6 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.101 ISF6_4831
-
 Ideonella sakaiensis
3.1.1.101 PETase
-
 Ideonella sakaiensis
3.1.1.102 ISF6_0224
-
 Ideonella sakaiensis
3.1.1.102 MHETase
-
 Ideonella sakaiensis
3.1.1.102 mono(2-hydroxyethyl) terephthalate hydrolase
-
 Ideonella sakaiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.102 31
-
 ethylene terephthalate pH 7.0, 30°C Ideonella sakaiensis