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Literature summary extracted from

  • Ishida, Y; Kuwahara, Y.; Dadashipour, M; Ina, A; Yamaguchi, T; Morita, M.; Ichiki, Y; Asano, Y
    A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase (2016), Sci. Rep., 6, 26998.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.49 8-hydroxyquinoline 1 mM, 56% residual activity Chamberlinius hualienensis
1.1.3.49 potassium cyanide 1 mM, 12.7% residual activity Chamberlinius hualienensis
1.1.3.49 Sodium azide 1 mM, 28.6% residual activity Chamberlinius hualienensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.49 2.1
-
 (R,S)-mandelonitrile pH 5.0, 25°C Chamberlinius hualienensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.3.49 ferric chloride 1 mM, 124% of initial activity Chamberlinius hualienensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.49 65000
-
 gel filtration Chamberlinius hualienensis
1.1.3.49 67000
-
-
 Chamberlinius hualienensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.49 Chamberlinius hualienensis A0A173MZJ8
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.3.49 glycoprotein sequence contains six predicted N-glycosylation sites at resiudes N57, N79, N94, N149, N406, and N491 Chamberlinius hualienensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.49 blood
-
 Chamberlinius hualienensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.49 (3E,5E)-2-hydroxydeca-3,5-dienenitrile + O2 10% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3E,5E)-2-oxodeca-3,5-dienenitrile + H2O2
-
 ?
1.1.3.49 (E)-2-hydroxy-4-phenylbut-3-enenitrile + O2 29% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3E)-2-oxo-4-phenylbut-3-enenitrile + H2O2
-
 ?
1.1.3.49 (R)-mandelonitrile + O2
-
 Chamberlinius hualienensis benzoyl cyanide + H2O2 enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile ?
1.1.3.49 (R,S)-mandelonitrile + O2
-
 Chamberlinius hualienensis benzoyl cyanide + H2O2
-
 ?
1.1.3.49 2-(3-bromophenyl)-2-hydroxyacetonitrile + O2 22% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (3-bromophenyl)(oxo)acetonitrile + H2O2
-
 ?
1.1.3.49 2-hydroxy-2-(2-methoxyphenyl)acetonitrile + O2 17% of the activity with (R)-mandelonitrile Chamberlinius hualienensis (2-methoxyphenyl)(oxo)acetonitrile + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.49 monomer 1 * 67000, SDS-PAGE, 1 * 62818, calculated Chamberlinius hualienensis

Synonyms

EC Number Synonyms Comment Organism
1.1.3.49 ChuaMOX
-
 Chamberlinius hualienensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.49 35
-
-
 Chamberlinius hualienensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.3.49 30
-
 90% of maximum activity Chamberlinius hualienensis
1.1.3.49 40
-
 90% of maximum activity Chamberlinius hualienensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.49 70
-
 1 h, complete loss of activity Chamberlinius hualienensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.49 4 4.5
-
 Chamberlinius hualienensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.3.49 3.5
-
 80% of maximum activity Chamberlinius hualienensis
1.1.3.49 5
-
 80% of maximum activity Chamberlinius hualienensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.49 8
-
 25-50 °C, 1 h, 80% residual activity Chamberlinius hualienensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.49 FAD sequence contains a FAD binding motif in the T22-S62 region Chamberlinius hualienensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.3.49 Chamberlinius hualienensis calculated
-
 6.3

General Information

EC Number General Information Comment Organism
1.1.3.49 physiological function the enzyme stoichiometrically catalyzes oxygen consumption and synthesis of benzoyl cyanide and hydrogen peroxide from mandelonitrile. The enzymatic activity is suppressed at a blood pH of 7, and the enzyme is segregated by membranes of defensive sacs from its substrate mandelonitrile which has a pH of 4.6, the optimum pH for ChuaMOX activity. Strong body muscle contractions are necessary for de novo synthesis of benzoyl cyanide Chamberlinius hualienensis