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Literature summary extracted from

  • Ferreira, E.R.; Horjales, E.; Bonfim-Melo, A.; Cortez, C.; da Silva, C.V.; De Groote, M.; Sobreira, T.J.; Cruz, M.C.; Lima, F.M.; Cordero, E.M.; Yoshida, N.; da Silveira, J.F.; Mortara, R.A.; Bahia, D.
    Unique behavior of Trypanosoma cruzi mevalonate kinase: A conserved glycosomal enzyme involved in host cell invasion and signaling (2016), Sci. Rep., 6, 24610.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.36 expressed in Escherichia coli BL21 cells Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.36 glycosome
-
 Trypanosoma cruzi 20015
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.36 34000
-
 2 * 34000, SDS-PAGE Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate Trypanosoma cruzi
-
 ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate Trypanosoma cruzi CL Brener
-
 ADP + (R)-5-phosphomevalonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.36 Trypanosoma cruzi Q4CLD0
-
-
2.7.1.36 Trypanosoma cruzi CL Brener Q4CLD0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.36 Ni-NTA column chromatography and Superdex 200 gel filtration Trypanosoma cruzi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.36 72.8
-
 at pH 7.2 and 37°C Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.36 ATP + (R)-mevalonate
-
 Trypanosoma cruzi ADP + (R)-5-phosphomevalonate
-
 ?
2.7.1.36 ATP + (R)-mevalonate
-
 Trypanosoma cruzi CL Brener ADP + (R)-5-phosphomevalonate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.36 homodimer 2 * 34000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.7.1.36 MVK
-
 Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.7.1.36 physiological function thebifunctional enzyme, in addition to playing a classical role in isoprenoid synthesis in lycosomes, it is secreted and modulates host cell signaling required for Trypanosoma cruzi invasion Trypanosoma cruzi