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Literature summary extracted from

  • Wang, Y.Y.; Zhang, X.S.; Luo, H.D.; Ren, N.N.; Jiang, X.H.; Jiang, H.; Li, Y.Q.
    Characterization of discrete phosphopantetheinyl transferases in Streptomyces tsukubaensis L19 unveils a complicate phosphopantetheinylation network (2016), Sci. Rep., 6, 24255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 DNA and amino acid sequence determination and analysis, recombinant expression of tagged isozyme in Escherichia coli Streptomyces tsukubensis
2.7.8.7 gene acpS, DNA and amino acid sequence determination and analysis, recombinant expression of tagged isozyme in Escherichia coli Streptomyces tsukubensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Streptomyces tsukubensis
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] Streptomyces tsukubensis L19
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Streptomyces tsukubensis A0A0X8EK72 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis A0A0Y0S258 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis A0A0Y0SAB4 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis A0A109R3N8 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis I2N4F3 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis L19 A0A0X8EK72 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis L19 A0A0Y0S258 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis L19 A0A0Y0SAB4 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis L19 A0A109R3N8 isolated from Yunnan, China
-
2.7.8.7 Streptomyces tsukubensis L19 I2N4F3 isolated from Yunnan, China
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant tagged isozyme from Escherichia coli by affinity chromatography Streptomyces tsukubensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Streptomyces tsukubensis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein]
-
 Streptomyces tsukubensis L19 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 ?
2.7.8.7 additional information substrate specificities of isozymes with different ACPs: stw ACP, an ACP in a type II PKS, is phosphopantetheinylated by three PPTases FKPPT1, FKPPT3, and FKACPS. sts FAS ACP, the ACP in fatty acid synthase (FAS), is phosphopantetheinylated by three PPTases FKPPT2, FKPPT3, and FKACPS. TcsA-ACP, an ACP involved in FK506 biosynthesis, is phosphopantetheinylated by two PPTases FKPPT3 and FKACPS. FkbPPCP, an PCP involved in FK506 biosynthesis, is phosphopantetheinylated by all of these five PPTases FKPPT1-4 and FKACPS Streptomyces tsukubensis ?
-
 ?
2.7.8.7 additional information substrate specificities of isozymes with different ACPs: stw ACP, an ACP in a type II PKS, is phosphopantetheinylated by three PPTases FKPPT1, FKPPT3, and FKACPS. sts FAS ACP, the ACP in fatty acid synthase (FAS), is phosphopantetheinylated by three PPTases FKPPT2, FKPPT3, and FKACPS. TcsA-ACP, an ACP involved in FK506 biosynthesis, is phosphopantetheinylated by two PPTases FKPPT3 and FKACPS. FkbPPCP, an PCP involved in FK506 biosynthesis, is phosphopantetheinylated by all of these five PPTases FKPPT1-4 and FKACPS Streptomyces tsukubensis L19 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.7 FKACPS
-
 Streptomyces tsukubensis
2.7.8.7 FKPPT1
-
 Streptomyces tsukubensis
2.7.8.7 FKPPT2
-
 Streptomyces tsukubensis
2.7.8.7 FKPPT3
-
 Streptomyces tsukubensis
2.7.8.7 FKPPT4
-
 Streptomyces tsukubensis
2.7.8.7 phosphopantetheinyl transferase
-
 Streptomyces tsukubensis
2.7.8.7 PPTase
-
 Streptomyces tsukubensis

General Information

EC Number General Information Comment Organism
2.7.8.7 evolution PPTases can be classified into three groups according to their structures. A group I PPTase, also named as an ACPS-type PPTase, consists of three identical peptide subunits with about 120 amino acid residues in each subunit. A group II PPTase, also named as a Sfp-type PPTase, consists of one peptide which is about twice the size of one group I PPTase subunit. A group III PPTase exists as a domain of a FAS or a PKS. FKPPT1, FKPPT2, FKPPT3, FKPPT4 contains three conserved motifs, PRWP, GID and FSAKESVYK, found in the Sfp-type PPTase motifs P1, P2 and P3, while FKACPS contains just last two conserved motifs found in ACPS-type PPTase. Thus, FKPPT1, FKPPT2, FKPPT3, and FKPPT4 belong to the Sfp-type PPTase group, and FKACPS belongs to the ACPS-type PPTase group, respectively Streptomyces tsukubensis
2.7.8.7 malfunction inactivation of FKPPT1 or FKPPT3 decreases the FK506 yield in Streptomyces tsukubaensis strain L19 Streptomyces tsukubensis
2.7.8.7 malfunction inactivation of FKPPT1 or FKPPT3 decreases the FK506 yield in Streptomyces tsukubaensis strain L19, and overexpression of FKPPT3 increases the FK506 production Streptomyces tsukubensis
2.7.8.7 physiological function phosphopantetheinyl transferases (PPTases) catalyze the phosphopantetheinylation of acyl carrier proteins (ACPs) in polyketide synthases (PKSs), peptidyl carrier proteins (PCPs) in nonribosomal peptide synthetases (NRPSs), and ACPs in fatty acid synthases (FASs) from inactive apo-forms into active holo-form. PPTases are essential to both primary metabolisms and secondary metabolisms. Phosphopantetheinylation of the ACP/PCP is involved in FK506 biosynthesis, FK506 (tacrolimus) is a clinical immunosuppressant widely used after allogeneic kidney, liver, and heart transplantations Streptomyces tsukubensis