EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.395 | expressed in Escherichia coli | [Clostridium] scindens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.395 | apoenzyme isoform BaiA2 and in complex with NAD+, sitting nano-drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.1M Tris-HCl pH 8.5, and 0.2 M sodium acetate | [Clostridium] scindens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.395 | E42A | the mutation improves specificity toward NADP+ by 10fold compared to the wild type enzyme | [Clostridium] scindens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.395 | 0.0011 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0013 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0015 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0018 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0033 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0056 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0067 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0083 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.0091 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.015 | - |
NADP+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.016 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.017 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.017 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.017 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.018 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.022 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.023 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.032 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.053 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.084 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.106 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.201 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.205 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.329 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.334 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.383 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.555 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.802 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.883 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 1.158 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.395 | [Clostridium] scindens | P19337 | - |
- |
1.1.1.395 | [Clostridium] scindens VPI 12708 | P19337 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.395 | Ni-NTA column chromatography | [Clostridium] scindens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.395 | 3-oxochenodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxochenodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxocholoyl CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxocholoyl CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxolithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
1.1.1.395 | 3-oxolithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
1.1.1.395 | chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens | 7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+ | - |
? | |
1.1.1.395 | chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens VPI 12708 | 7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+ | - |
? | |
1.1.1.395 | chenodeoxycholoyl-CoA + NADP+ | - |
[Clostridium] scindens | 3-oxochenodeoxycholoyl-CoA + NADPH + H+ | - |
? | |
1.1.1.395 | chenodeoxycholyl CoA + NAD+ | - |
[Clostridium] scindens | 3-oxo-chenodeoxycholy-CoA + NADH + H+ | - |
? | |
1.1.1.395 | cholic acid + NAD+ | - |
[Clostridium] scindens | 3-oxocholic acid + NADH + H+ | - |
? | |
1.1.1.395 | choloyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxocholoyl-CoA + NADH + H+ | - |
? | |
1.1.1.395 | deoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxodeoxycholoyl-CoA + NADH + H+ | - |
? | |
1.1.1.395 | glycocholic acid + NAD+ | - |
[Clostridium] scindens | N-[7alpha,12-alpha-3,24-dioxo-5beta-cholan-24-yl]glycine + NADH + H+ | - |
? | |
1.1.1.395 | lithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxolithocholoyl-CoA + NADH + H+ | - |
? | |
1.1.1.395 | taurocholic acid + NAD+ | - |
[Clostridium] scindens | 3-oxotaurocholic acid + NADH + H+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.395 | 3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase | - |
[Clostridium] scindens |
1.1.1.395 | baiA1 | isoform | [Clostridium] scindens |
1.1.1.395 | baiA2 | isoform | [Clostridium] scindens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.395 | 0.025 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.05 | - |
NADP+ | mutantE42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.055 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.089 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.09 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.11 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.12 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.14 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.145 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.15 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.15 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.16 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.17 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.17 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.18 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.2 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.25 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.27 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.3 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.32 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.4 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.4 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.42 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.55 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.7 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.73 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.73 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.82 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.87 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 1.23 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.395 | NAD+ | preferred cofactor | [Clostridium] scindens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.395 | 0.12 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.13 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.17 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.23 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.3 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.45 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.72 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 0.73 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 1 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 1 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 3.3 | - |
NADP+ | mutantE42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 5.22 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 8.7 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 9.32 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 10.18 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 11.6 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 13.17 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 15.4 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 17.7 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 18.5 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 33.3 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 54.2 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 60.4 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 76.85 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 84.3 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 93.58 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 101.7 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 120.5 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 131 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.1.1.395 | 151.5 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens |