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Literature summary extracted from

  • Zhang, F.; Xu, Y.; Zhang, Z.
    Heterologous expression, purification, crystallization and preliminary X-ray diffraction analysis of KDO8P synthase from Arabidopsis thaliana (2014), Protein Expr. Purif., 101, 133-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.55 gene kdsA1, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana
2.5.1.55 gene kdsA2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.55 purified recombinant enzyme, hanging-drop vapor diffusion method, mixing of 300 nl of 10 mg/ml protein in 30 mM Tris-HCl, pH 8.0, 200 mM NaCl with 300 nl reservoir solution containing 28% v/v PEG 400, 0.1 M HEPES, pH 7.5, and 0.2 M CaCl2, X-ray diffraction structure determinatin and analysis Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 33500
-
-
 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Arabidopsis thaliana
-
 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Arabidopsis thaliana Q6NQL4 gene kdsA2; gene kdsA2
-
2.5.1.55 Arabidopsis thaliana Q9AV97 gene kdsA1; gene kdsA1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Arabidopsis thaliana 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 dimer 2 * 33545, sequence calculation, 2 * 33500, recombinant His-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-octulosonate 8-phosphate synthase
-
 Arabidopsis thaliana
2.5.1.55 3-eoxy-D-manno-octulosonate 8-phosphate synthase
-
 Arabidopsis thaliana
2.5.1.55 KDO8PS
-
 Arabidopsis thaliana
2.5.1.55 KdsA1
-
 Arabidopsis thaliana
2.5.1.55 KdsA2
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.55 37
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.55 7.5
-
 assay at Arabidopsis thaliana