BRENDA - Enzyme Database show

Potent sensitisation of cancer cells to anticancer drugs by a quadruple mutant of the human deoxycytidine kinase

Coulibaly, S.T.; Rossolillo, P.; Winter, F.; Kretzschmar, F.K.; Braye, M.; Martin, D.P.; Lener, D.; Negroni, M.; PLoS ONE 10, e0140741 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.74
expressed in Escherichia coli BL21 DE3 pLysE cells
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00542
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.0282
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.0467
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + deoxycytidine
Homo sapiens
-
NDP + dCMP
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.74
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.74
His-Trap TM column chromatography and Sephacryl S-200 gel filtration
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
HEK-293T cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + cytarabine
-
739431
Homo sapiens
?
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
739431
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + gemcitabine
-
739431
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.74
homodimer
-
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.035
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.38
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.57
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
expressed in Escherichia coli BL21 DE3 pLysE cells
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.00542
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.0282
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.0467
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.74
NTP + deoxycytidine
Homo sapiens
-
NDP + dCMP
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.74
His-Trap TM column chromatography and Sephacryl S-200 gel filtration
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.74
HEK-293T cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.74
NTP + cytarabine
-
739431
Homo sapiens
?
-
-
-
?
2.7.1.74
NTP + deoxycytidine
-
739431
Homo sapiens
NDP + dCMP
-
-
-
?
2.7.1.74
NTP + gemcitabine
-
739431
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.74
homodimer
-
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.74
0.035
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.38
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
0.57
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.74
6.46
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
12.21
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
13.48
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.7.1.74
6.46
-
deoxycytidine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
12.21
-
gemcitabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens
2.7.1.74
13.48
-
cytarabine
wild type enzyme, pH and temperature not specified in the publication
Homo sapiens