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Literature summary extracted from

  • Coulibaly, S.T.; Rossolillo, P.; Winter, F.; Kretzschmar, F.K.; Braye, M.; Martin, D.P.; Lener, D.; Negroni, M.
    Potent sensitisation of cancer cells to anticancer drugs by a quadruple mutant of the human deoxycytidine kinase (2015), PLoS ONE, 10, e0140741.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.74 expressed in Escherichia coli BL21 DE3 pLysE cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 0.00542
-
 deoxycytidine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 0.0282
-
 cytarabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 0.0467
-
 gemcitabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.74 NTP + deoxycytidine Homo sapiens
-
 NDP + dCMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.74 His-Trap TM column chromatography and Sephacryl S-200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.74 HEK-293T cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 NTP + cytarabine
-
 Homo sapiens ?
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Homo sapiens NDP + dCMP
-
 ?
2.7.1.74 NTP + gemcitabine
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.74 homodimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.74 dCK
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.74 0.035
-
 deoxycytidine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 0.38
-
 cytarabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 0.57
-
 gemcitabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.74 6.46
-
 deoxycytidine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 12.21
-
 gemcitabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens
2.7.1.74 13.48
-
 cytarabine wild type enzyme, pH and temperature not specified in the publication Homo sapiens