Literature summary extracted from
Rossi, A.H.; Farias, A.; Fernandez, J.E.; Bonomi, H.R.; Goldbaum, F.A.; Berguer, P.M.
Brucella spp. lumazine synthase induces a TLR4-mediated protective response against B16 melanoma in mice (2015), PLoS ONE, 10, e0126827.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.5.1.78 |
medicine |
partially protective effect of enzyme BLS against B16 melanoma in mice generating a protective response at the first stages of tumor growth but not at later stages, therapeutic effect of BLS vaccination |
Brucella sp. |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.5.1.78 |
recombinant expression of enzyme BLS in Escherichia coli strain BL21 (DE3) |
Brucella sp. |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.5.1.78 |
additional information |
for evaluation of the effectiveness of enzyme BLS as a preventive vaccine, C57BL/6J mice are immunized with BLS or BLS-OVA, subcutaneous inoculation with B16-OVA melanoma, and analysis of the effect induced by BLS in TLR4-expressing B16 melanoma cells. BLS or BLS-OVA induce a significant inhibition of tumor growth, survival is increased, and 50% of mice immunized with the highest dose of BLS do not develop visible tumors |
Brucella sp. |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.5.1.78 |
Brucella sp. |
P61711 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.5.1.78 |
recombinant enzyme BLS from Escherichia coli strain BL21 (DE3) by dialysis, anion exchange chromatography, and gel filtration |
Brucella sp. |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.5.1.78 |
decamer |
- |
Brucella sp. |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.5.1.78 |
BLS |
- |
Brucella sp. |
2.5.1.78 |
lumazine synthase |
- |
Brucella sp. |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.5.1.78 |
physiological function |
lumazine synthase (BLS) is a highly immunogenic decameric protein which can accept the fusion of foreign proteins at its ten N-termini. These chimeras are very efficient to elicit systemic and oral immunity without adjuvants. BLS signaling via Toll-like receptor 4 (TLR4) regulates innate and adaptive immune responses, inducing dendritic cell maturation and CD8+ T-cell cytotoxicity |
Brucella sp. |