BRENDA - Enzyme Database

Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis

Muramoto, T.; Tsurui, N.; Terry, M.; Yokota, A.; Kohchi, T.; Plant Physiol. 130, 1958-1966 (2002)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.15.20
4,5-dihydroxy-1,3-benzene disulfonic acid
-
Arabidopsis thaliana
1.14.15.20
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
significantly increases enzyme activity
Arabidopsis thaliana
1.14.15.20
dehydroascorbate
-
Arabidopsis thaliana
1.14.15.20
desferrioxamine
-
Arabidopsis thaliana
1.14.15.20
isoascorbate
-
Arabidopsis thaliana
1.14.15.20
L-ascorbate
the enzyme requires an iron chelator and second reductant, such as L-ascorbate, for full activity
Arabidopsis thaliana
1.14.15.20
o-phenanthroline
-
Arabidopsis thaliana
1.14.15.20
Trolox
-
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.20
expressed in Escherichia coli BL21 cells
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.20
0.0013
-
hemin
at pH 7.2 and 25°C
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.20
27000
-
x * 27000, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
1.14.15.20
53000
-
gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.20
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.20
Q sepharose column chromatography
Arabidopsis thaliana
Storage Stability
EC Number
Storage Stability
Organism
1.14.15.20
-30°C, in 50 mM HEPES-NaOH (pH 7.2) with 40% (v/v) glycerol, at least 3 months, no loss of activity
Arabidopsis thaliana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
739342
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 27000, recombinant enzyme, SDS-PAGE; x * 53000, GST-tagged enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.20
50
-
-
Arabidopsis thaliana
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.20
7.2
-
-
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.20
heme
the enzyme forms a 1:1 complex with heme
Arabidopsis thaliana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.15.20
4,5-dihydroxy-1,3-benzene disulfonic acid
-
Arabidopsis thaliana
1.14.15.20
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
significantly increases enzyme activity
Arabidopsis thaliana
1.14.15.20
dehydroascorbate
-
Arabidopsis thaliana
1.14.15.20
desferrioxamine
-
Arabidopsis thaliana
1.14.15.20
isoascorbate
-
Arabidopsis thaliana
1.14.15.20
L-ascorbate
the enzyme requires an iron chelator and second reductant, such as L-ascorbate, for full activity
Arabidopsis thaliana
1.14.15.20
o-phenanthroline
-
Arabidopsis thaliana
1.14.15.20
Trolox
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.20
expressed in Escherichia coli BL21 cells
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.20
heme
the enzyme forms a 1:1 complex with heme
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.20
0.0013
-
hemin
at pH 7.2 and 25°C
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.20
27000
-
x * 27000, recombinant enzyme, SDS-PAGE
Arabidopsis thaliana
1.14.15.20
53000
-
gel filtration
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.20
Q sepharose column chromatography
Arabidopsis thaliana
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.15.20
-30°C, in 50 mM HEPES-NaOH (pH 7.2) with 40% (v/v) glycerol, at least 3 months, no loss of activity
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
739342
Arabidopsis thaliana
biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 27000, recombinant enzyme, SDS-PAGE; x * 53000, GST-tagged enzyme, SDS-PAGE
Arabidopsis thaliana
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.20
50
-
-
Arabidopsis thaliana
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.20
7.2
-
-
Arabidopsis thaliana