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Literature summary extracted from

  • Kahn, R.A.; Bak, S.; Svendsen, I.; Halkier, B.A.; Moller, B.L.
    Isolation and reconstitution of cytochrome P450ox and in vitro reconstitution of the entire biosynthetic pathway of the cyanogenic glucoside dhurrin from sorghum (1997), Plant Physiol., 115, 1661-1670.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.36 biotechnology in vitro reconstitution of the entire dhurrin biosynthetic pathway from tyrosine is accomplished by the insertion of CYP79 (tyrosine N-hydroxylase), P450ox, and NADPH-P450 oxidoreductase in lipid micelles in the presence of uridine diphosphate glucose glucosyltransferase Sorghum bicolor
1.14.14.37 biotechnology in vitro reconstitution of the entire dhurrin biosynthetic pathway from tyrosine is accomplished by the insertion of CYP79 (tyrosine N-hydroxylase), P450ox, and NADPH-P450 oxidoreductase in lipid micelles in the presence of uridine diphosphate glucose glucosyltransferase Sorghum bicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.14.37 55000
-
 x * 55000, SDS-PAGE Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.36 Sorghum bicolor Q43135
-
-
1.14.14.37 Sorghum bicolor O48958
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.14.37 reconstitution with NADPH-P450 oxidoreductase in micelles of L-alpha-dilauroyl phosphatidylcholine Sorghum bicolor

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.37 (E)-4-Hydroxyphenylacetaldehyde oxime
-
 Sorghum bicolor (Z)-4-Hydroxyphenylacetaldehyde oxime
-
 ?
1.14.14.37 (E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?
1.14.14.37 (Z)-4-hydroxyphenylacetaldehyde oxime
-
 Sorghum bicolor 4-hydroxyphenylacetonitrile + H2O
-
 ?
1.14.14.37 4-hydroxyphenylacetonitrile + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.37 ? x * 55000, SDS-PAGE Sorghum bicolor

General Information

EC Number General Information Comment Organism
1.14.14.37 physiological function the enzyme is a multifunctional P450 catalyzing dehydration of (Z)-4-hydroxyphenylacetaldoxime to 4-hydroxyphenylacetonitrile and C-hydroxylation of 4-hydroxyphenylacetonitrile during biosynthesis of the cyanogenic glucoside beta-D-glucopyranosyloxy-(S)-4-hydroxymandelonitrile (dhurrin) Sorghum bicolor