BRENDA - Enzyme Database

Phytobilin biosynthesis: Cloning and expression of a gene encoding soluble ferredoxin-dependent heme oxygenase from Synechocystis sp. PCC 6803

Cornejo, J.; Willows, R.; Beale, S.; Plant J. 15, 99-107 (1998)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.20
expressed in Escherichia coli BL21(DE3) cells
Synechocystis sp. PCC 6803
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.20
Sn-protoporphyrin IX
25% inhibition at 0.02 mM
Synechocystis sp. PCC 6803
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.20
31000
-
-
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechocystis sp. PCC 6803
-
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.20
Synechocystis sp. PCC 6803
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.20
partial purification by nickel chelating column chromatography
Synechocystis sp. PCC 6803
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
739302
Synechocystis sp. PCC 6803
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 28540, isoform HO2, calculated from amino acid sequence; x * 31000, isoform HO2, SDS-PAGE
Synechocystis sp. PCC 6803
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.20
expressed in Escherichia coli BL21(DE3) cells
Synechocystis sp. PCC 6803
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.20
Sn-protoporphyrin IX
25% inhibition at 0.02 mM
Synechocystis sp. PCC 6803
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.20
31000
-
-
Synechocystis sp. PCC 6803
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.20
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Synechocystis sp. PCC 6803
-
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.20
partial purification by nickel chelating column chromatography
Synechocystis sp. PCC 6803
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.20
mesoheme + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
739302
Synechocystis sp. PCC 6803
mesobiliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.20
?
x * 28540, isoform HO2, calculated from amino acid sequence; x * 31000, isoform HO2, SDS-PAGE
Synechocystis sp. PCC 6803
Expression
EC Number
Organism
Commentary
Expression
1.14.15.20
Synechocystis sp. PCC 6803
enzyme expression increases about 7fold during incubation of cells for 72 h in iron-deficient medium
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.15.20
Synechocystis sp. PCC 6803
enzyme expression increases about 7fold during incubation of cells for 72 h in iron-deficient medium
up