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Literature summary extracted from

  • Nowicki, E.M.; OBrien, J.P.; Brodbelt, J.S.; Trent, M.S.
    Extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system (2015), Mol. Microbiol., 97, 166-178.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.43 gene eptA, quantitative and semi-quantitative reverse-transcriptase (RT) PCR enzyme expression analysis Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.43 additional information lipid A prepared from the PA14DELTAeptAPa mutant shows no phosphatidylethanolamine modification when Zn2+ is added to the media Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.43 Zn2+ extracellular zinc induces phosphoethanolamine addition to Pseudomonas aeruginosa lipid A via the ColRS two-component system Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.43 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A Pseudomonas aeruginosa
-
 alpha-Kdo-(2->4)-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Pseudomonas aeruginosa
-
 diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.43 Pseudomonas aeruginosa
-
-
-
2.7.8.43 Pseudomonas aeruginosa A0A069Q704 gene eptA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.43 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A
-
 Pseudomonas aeruginosa alpha-Kdo-(2->4)-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A
-
 Pseudomonas aeruginosa diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Pseudomonas aeruginosa lipid A, enzyme EptAPa-dependent addition of pEtN to the 4' phosphate group of BN2 and PA14 lipid A Pseudomonas aeruginosa diacylglycerol + lipid A 4'-(2-aminoethyl diphosphate)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.43 arnT
-
 Pseudomonas aeruginosa
2.4.2.53 EptA
-
-
2.4.2.53 PA14_39020
-
-
2.7.8.43 EptAPa
-
 Pseudomonas aeruginosa
2.7.8.43 pEtN transferase
-
 Pseudomonas aeruginosa

Expression

EC Number Organism Comment Expression
2.4.2.43 Pseudomonas aeruginosa 2 mM Zn2+ downregulates the enzyme transcription by over 4fold down
2.7.8.43 Pseudomonas aeruginosa transcription of the enzyme eptAPa is regulated by zinc via the ColRS two-component system. The two-component system response regulator ColR activates eptAPa transcription. Transcription of eptAPa is induced by 2 mM ZnSO4 approximately 21fold additional information

General Information

EC Number General Information Comment Organism
2.7.8.43 malfunction deletion of colR and of eptA results in loss of Zn2+-induced phosphatidylethanolamine modification of Pseudomonas aeruginosa lipid A. colR deletion mutant complementation restores Zn2+-dependent eptAPa transcription by more than fourfold Pseudomonas aeruginosa
2.7.8.43 physiological function Gram-negative bacteria survive harmful environmental stressors by modifying their outer membrane. Much of this protection is afforded upon remodeling of the lipid A region of the major surface molecule lipopolysaccharide. Addition of cationic substituents, such as 4-amino-4-deoxy-L-arabinose (L-Ara4N) and phosphoethanolamine (pEtN) at the lipid A phosphate groups, is often induced in response to specific environmental flux stabilizing the outer membrane. ColR specifically induces pEtN addition to lipid A in lieu of L-Ara4N when Zn2+ is present Pseudomonas aeruginosa