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Literature summary extracted from

  • Asencion Diez, M.D.; Demonte, A.M.; Guerrero, S.A.; Ballicora, M.A.; Iglesias, A.A.
    The ADP-glucose pyrophosphorylase from Streptococcus mutans provides evidence for the regulation of polysaccharide biosynthesis in Firmicutes (2013), Mol. Microbiol., 90, 1011-1027.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.27 D-fructose 1,6-bisphosphate activates GlgC by twofold, but has no effect on GlgC/GlgD complex. In the presence of D-fructose 1,6-bisphosphate GlgC becomes sensitive to inhibitors phosphoenolpyruvate and inorganic salts Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.27 ammonium sulfate inhibits both subunit GlgC and GlgC/GlgD complex Streptococcus mutans
2.7.7.27 KCl inhibits GlgC/GlgD complex, no effect on subunit GlgC alone Streptococcus mutans
2.7.7.27 NaCl inhibits GlgC/GlgD complex, no effect on subunit GlgC alone Streptococcus mutans
2.7.7.27 phosphate inhibits both subunit GlgC and GlgC/GlgD complex Streptococcus mutans
2.7.7.27 phosphoenolpyruvate inhibitory to GlgC/GlgD complex, inhibitory to subunit GlgC only in presence of fructose 1,6-bisphosphate Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.27 0.07
-
 alpha-D-glucose 1-phosphate S0.5 value, Hill coefficient 1.2, GlgC/GlgD complex, pH 8.0, 37°C Streptococcus mutans
2.7.7.27 0.37
-
 ATP S0.5 value, Hill coefficient 1.5, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C Streptococcus mutans
2.7.7.27 1.18
-
 ATP S0.5 value, Hill coefficient 3.7, GlgC/GlgD complex, pH 8.0, 37°C Streptococcus mutans
2.7.7.27 2.04
-
 alpha-D-glucose 1-phosphate S0.5 value, Hill coefficient 1.7, subunit GlgC in presence of fructose 1,6-bisphosphate, pH 8.0, 37°C Streptococcus mutans
2.7.7.27 3.42
-
 ATP S0.5 value, Hill coefficient 1.2, subunit GlgC, pH 8.0, 37°C Streptococcus mutans
2.7.7.27 3.44
-
 alpha-D-glucose 1-phosphate S0.5 value, Hill coefficient 0.9, subunit GlgC, pH 8.0, 37°C Streptococcus mutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.27 Mg2+ subunit GlgC, S0.5 value 1.55 mM and Hill coefficient 1.7, complex of GlgC/GlgD, S0.5 value 2.36, hill coefficient 1.6 Streptococcus mutans

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.27 Streptococcus mutans M9TIK1 and M9TG57 M9TIK1 i.e. subunit GlgC, M9TG57 i.e. subunit GlgD
-
2.7.7.27 Streptococcus mutans ATCC 25175 M9TIK1 and M9TG57 M9TIK1 i.e. subunit GlgC, M9TG57 i.e. subunit GlgD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Streptococcus mutans diphosphate + ADP-glucose
-
 ?
2.7.7.27 ATP + alpha-D-glucose 1-phosphate
-
 Streptococcus mutans ATCC 25175 diphosphate + ADP-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.27 GlgC
-
 Streptococcus mutans
2.7.7.27 GlgD
-
 Streptococcus mutans

General Information

EC Number General Information Comment Organism
2.7.7.27 physiological function when expressed individually, subunit GlgC has ADP-Glc diphosphorylase activity, whereas subunit GlgD is inactive. The coexpressed GlgC/GlgD protein is one order of magnitude more active than subunit GlgC alone. Fructose-1,6-bis-phosphate activates GlgC by twofold, but has no effect on GlgC/GlgD. Phosphoenolpyruvate and inorganic salts inhibit GlgC/GlgD without affecting GlgC. In the presence of fructose-1,6-bis-phosphate GlgC acquires a GlgC/GlgD-like behaviour, becoming sensitive to the stated inhibitors Streptococcus mutans