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Literature summary extracted from

  • He, Y.; Yam, C.; Pomraning, K.; Chin, J.S.; Yew, J.Y.; Freitag, M.; Oliferenko, S.
    Increase in cellular triacylglycerol content and emergence of large ER-associated lipid droplets in the absence of CDP-DG synthase function (2014), Mol. Biol. Cell, 25, 4083-4095.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.41 C1226T/C1227T/P363S construction of loss-of-function mutation in CDP-DG synthase in Schizosaccharomyces japonicus bbl1 mutant cells, the mutation triggers abnormal lipid droplet biogenesis and triacylglycerol accumulation. The P363S mutation in the Cds1 enzyme leads to a reduction in its CDP-DG synthase activity and potential shunting of a bulk of cellular phosphatidate into triacylglycerol biosynthesis through the DG pathway. The cds1bbl1 mutation is recessive, since introduction of the wild-type gene as an extra copy fully rescues the phenotype of bbl1 cells. Mutant bbl1 phenotype, overview Schizosaccharomyces japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.41 endoplasmic reticulum
-
 Schizosaccharomyces pombe 5783
-
2.7.7.41 endoplasmic reticulum
-
 Schizosaccharomyces japonicus 5783
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.41 CTP + phosphatidate Schizosaccharomyces pombe
-
 diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate Schizosaccharomyces japonicus
-
 diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate Schizosaccharomyces pombe 972
-
 diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate Schizosaccharomyces japonicus yFS275
-
 diphosphate + CDP-diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.41 Schizosaccharomyces japonicus
-
-
-
2.7.7.41 Schizosaccharomyces japonicus B6JVL2
-
-
2.7.7.41 Schizosaccharomyces japonicus yFS275 B6JVL2
-
-
2.7.7.41 Schizosaccharomyces pombe Q9P381
-
-
2.7.7.41 Schizosaccharomyces pombe 972 Q9P381
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces pombe diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces japonicus diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces pombe 972 diphosphate + CDP-diacylglycerol
-
 ?
2.7.7.41 CTP + phosphatidate
-
 Schizosaccharomyces japonicus yFS275 diphosphate + CDP-diacylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.41 CDP-DG synthase
-
 Schizosaccharomyces pombe
2.7.7.41 CDP-DG synthase
-
 Schizosaccharomyces japonicus
2.7.7.41 CDS1
-
 Schizosaccharomyces pombe
2.7.7.41 CDS1
-
 Schizosaccharomyces japonicus
2.7.7.41 SJAG_00426
-
 Schizosaccharomyces japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.41 36
-
 assay at Schizosaccharomyces pombe
2.7.7.41 36
-
 assay at Schizosaccharomyces japonicus

General Information

EC Number General Information Comment Organism
2.7.7.41 evolution Cds1 is an evolutionarily conserved protein Schizosaccharomyces pombe
2.7.7.41 evolution Cds1 is an evolutionarily conserved protein Schizosaccharomyces japonicus
2.7.7.41 malfunction cycling bbl1 mutant cells deficient in the function of the endoplasmic reticlum-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the endoplasmic reticlum membranes. These unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycer­ol-rich lifestyle reliant on the Kennedy pathway. The bbl1 mutant enzyme is catalytically deficient, penotype, overview Schizosaccharomyces japonicus
2.7.7.41 malfunction cycling cells deficient in the function of the endoplasmic reticlum-resident CDP-DG synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the endoplasmic reticlum membranes. These unusual structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion. Interfering with the CDP-DG route of phosphatidic acid utilization rewires cellular metabolism to adopt a triacylglycer­ol-rich lifestyle reliant on the Kennedy pathway Schizosaccharomyces pombe
2.7.7.41 physiological function cycling cells deficient in the function of the ER-resident CDP-diacylglycerol synthase Cds1 exhibit markedly increased triacylglycerol content and assemble large lipid droplets closely associated with the ER membranes. These structures recruit the triacylglycerol synthesis machinery and grow by expansion rather than by fusion Schizosaccharomyces japonicus
2.7.7.41 physiological function CDP-DG synthase is responsible for a rate-limiting step in the production of phospholipids. It catalyzes condensation of phosphatidate and cytidine triphosphate, releasing the CDP-diacylglycerol and diphosphate products Schizosaccharomyces pombe
2.7.7.41 physiological function CDP-DG synthase is responsible for a rate-limiting step in the production of phospholipids. It catalyzes condensation of phosphatidate and cytidine triphosphate, releasing the CDP-diacylglycerol and diphosphate products Schizosaccharomyces japonicus