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Literature summary extracted from

  • Klemke, F.; Beyer, G.; Sawade, L.; Saitov, A.; Korte, T.; Maldener, I.; Lockau, W.; Nuernberg, D.J.; Volkmer, T.
    All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120 (2014), Microbiology, 160, 2807-2819.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.63 expressed in Escherichia coli BL21(DE3) cells Anabaena sp.
2.7.1.63 expression in Escherichia coli Nostoc sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.63 0.00176
-
 (phosphate)45 pH 7.5, 28°C Nostoc sp.
2.7.1.63 0.00176
-
 (Phosphate)n at pH 7.5 and 28°C Anabaena sp.
2.7.1.63 0.118
-
 D-glucose pH 7.5, 28°C Nostoc sp.
2.7.1.63 0.118
-
 D-glucose at pH 7.5 and 28°C Anabaena sp.
2.7.1.63 24.3
-
 D-mannose K0.5 value, pH 7.5, 28°C Nostoc sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.63 additional information enzyme is strictly dependent on the presence of divalent cations Nostoc sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.63 26000
-
-
 Nostoc sp.
2.7.1.63 26000
-
 x * 26000, SDS-PAGE Anabaena sp.
2.7.1.63 26600
-
-
 Nostoc sp.
2.7.1.63 26600
-
-
 Anabaena sp.
2.7.1.63 39000
-
 gel filtration Nostoc sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose Anabaena sp.
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose Anabaena sp. PCC 7120
-
 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + mannose Anabaena sp.
-
 (phosphate)n-1 + mannose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + mannose Anabaena sp. PCC 7120
-
 (phosphate)n-1 + mannose 6-phosphate
-
 ?
2.7.1.63 additional information Anabaena sp. no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate ?
-
 ?
2.7.1.63 additional information Anabaena sp. PCC 7120 no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Anabaena sp. Q8YX46
-
-
2.7.1.63 Anabaena sp. PCC 7120 Q8YX46
-
-
2.7.1.63 Nostoc sp. Q8YX46
-
-
2.7.1.63 Nostoc sp. PCC 7120 Q8YX46
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63 glutathione Sepharose column chromatography, and gel filtration Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)45 + D-glucose
-
 Nostoc sp. (phosphate)44 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)45 + D-glucose
-
 Nostoc sp. PCC 7120 (phosphate)44 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)45 + D-mannose
-
 Nostoc sp. (phosphate)44 + D-mannose 6-phosphate
-
 ?
2.7.1.63 (phosphate)45 + D-mannose
-
 Nostoc sp. PCC 7120 (phosphate)44 + D-mannose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Anabaena sp. (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Anabaena sp. PCC 7120 (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + mannose
-
 Anabaena sp. (phosphate)n-1 + mannose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + mannose
-
 Anabaena sp. PCC 7120 (phosphate)n-1 + mannose 6-phosphate
-
 ?
2.7.1.63 additional information no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate Anabaena sp. ?
-
 ?
2.7.1.63 additional information enzyme requires polyphosphate to confer its enzymic activity. The reaction follows a bi-bi ping-pong mechanism. No substrates: ATP, GTP, UTP, CTP, ADP, AMP, diphosphate Nostoc sp. ?
-
 ?
2.7.1.63 additional information no activity with ATP, GTP, UTP, CTP, ADP, AMP, and diphosphate Anabaena sp. PCC 7120 ?
-
 ?
2.7.1.63 additional information enzyme requires polyphosphate to confer its enzymic activity. The reaction follows a bi-bi ping-pong mechanism. No substrates: ATP, GTP, UTP, CTP, ADP, AMP, diphosphate Nostoc sp. PCC 7120 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.63 ? x * 26000, SDS-PAGE Anabaena sp.
2.7.1.63 ? x * 26600, calculated from amino acid sequence Anabaena sp.
2.7.1.63 dimer or monomer, 2 * 26000, SDS-PAGE, 2 * 26600, calculated Nostoc sp.
2.7.1.63 monomer or dimer, 1 * 26000, SDS-PAGE, 1 * 26600, calculated Nostoc sp.

Synonyms

EC Number Synonyms Comment Organism
2.7.1.63 All1371
-
 Anabaena sp.
2.7.1.63 All1371
-
 Nostoc sp.
2.7.1.63 polyphosphate glucokinase
-
 Anabaena sp.
2.7.1.63 polyphosphate-dependent glucokinase
-
 Anabaena sp.
2.7.1.63 PPGK
-
 Anabaena sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.63 0.21
-
 D-mannose pH 7.5, 28°C Nostoc sp.
2.7.1.63 0.21
-
 mannose at pH 7.5 and 28°C Anabaena sp.
2.7.1.63 48.2
-
 (phosphate)45 pH 7.5, 28°C Nostoc sp.
2.7.1.63 48.2
-
 D-glucose at pH 7.5 and 28°C Anabaena sp.
2.7.1.63 48.2
-
 (Phosphate)n at pH 7.5 and 28°C Anabaena sp.

General Information

EC Number General Information Comment Organism
2.7.1.63 physiological function a mutant strain lacking enzymatic activity is impaired under nitrogen-fixing conditions Nostoc sp.