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Literature summary extracted from

  • Nino, C.H.; Forero-Baena, N.; Contreras, L.E.; Sanchez-Lancheros, D.; Figarella, K.; Ramirez, M.H.
    Identification of the nicotinamide mononucleotide adenylyltransferase of Trypanosoma cruzi (2015), Mem. Inst. Oswaldo Cruz, 110, 890-897.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 gene TcCLB.507047.170 or Tc00.1047053507047.170, DNA and amino acid sequence determination and analysis from genomic DNA, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.1 Mg2+ required Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Trypanosoma cruzi
-
 diphosphate + NAD+
-
 ?
2.7.7.1 ATP + nicotinamide ribonucleotide Trypanosoma cruzi CL Brener
-
 diphosphate + NAD+
-
 ?
2.7.7.1 additional information Trypanosoma cruzi bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 ?
-
 ?
2.7.7.1 additional information Trypanosoma cruzi CL Brener bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Trypanosoma cruzi Q4DPR9 gene TcCLB.507047.170 or Tc00.1047053507047.170
-
2.7.7.1 Trypanosoma cruzi CL Brener Q4DPR9 gene TcCLB.507047.170 or Tc00.1047053507047.170
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.1 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Trypanosoma cruzi diphosphate + NAD+
-
 ?
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Trypanosoma cruzi CL Brener diphosphate + NAD+
-
 ?
2.7.7.1 additional information bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 Trypanosoma cruzi ?
-
 ?
2.7.7.1 additional information the enzyme shows activity for both NAMN and NMN, substrates used by NMNAT in the presence of ATP to form NAD+ and NAAD, respectively Trypanosoma cruzi ?
-
 ?
2.7.7.1 additional information bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 Trypanosoma cruzi CL Brener ?
-
 ?
2.7.7.1 additional information the enzyme shows activity for both NAMN and NMN, substrates used by NMNAT in the presence of ATP to form NAD+ and NAAD, respectively Trypanosoma cruzi CL Brener ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.1 More enzyme TcNMNAT secondary structure prediction and tertiary structure model, overview Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 nicotinamide mononucleotide adenylyltransferase
-
 Trypanosoma cruzi
2.7.7.1 NMNAT
-
 Trypanosoma cruzi
2.7.7.1 Tc00.1047053507047.170
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.1 37
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.1 7.4
-
 assay at Trypanosoma cruzi

General Information

EC Number General Information Comment Organism
2.7.7.1 metabolism NAD+ biosynthesis is performed by de novo and salvage pathways, which converge on the step that is catalysed by the enzyme nicotinamide mononucleotide adenylyltransferase, NMNAT Trypanosoma cruzi