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Literature summary extracted from

  • Zaslona, H.; Trusek-Holownia, A.; Radosinski, L.; Hennig, J.
    Optimization and kinetic characterization of recombinant 1,3-beta-glucanase production in Escherichia coli K-12 strain BL21/pETSD10 - a bioreactor scale study (2015), Lett. Appl. Microbiol., 61, 36-43.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.39 synthesis heterologous 1,3-beta-glucanase production in Escherichia coli is favoured with moderate culture aeration (0.7-0.9 vvm) and moderate stirring (125–150 rev/min) Solanum tuberosum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Escherichia coli Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 36500
-
 x * 36500, SDS-PAGE Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Solanum tuberosum Q70C53 cv. Desiree
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 252
-
 enzyme isolated from culture with aeration at 135 rev/min and stirring at 0.8 vvm, pH 5.6, 28°C Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
3.2.1.39 at 28°C, pH 5.6, half-life is 202 h Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 carboxymethyl curdlan + H2O
-
 Solanum tuberosum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 36500, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 GLUB20-2
-
 Solanum tuberosum