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Literature summary extracted from

  • Kim, E.J.; Kim, H.; Lee, J.K.
    The photoheterotrophic growth of bacteriochlorophyll synthase-deficient mutant of Rhodobacter sphaeroides is restored by I44F mutant chlorophyll synthase of Synechocystis sp. PCC 6803 (2016), J. Microbiol. Biotechnol., 26, 959-966.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.B43 gene bchG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Cereibacter sphaeroides
2.5.1.62 gene chlG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), functional recombinant expression of mutant I44F in Rhodobacter sphaeroides photosynthesis-deficient mutant strain BG1 that lacks a functional bchG gene encoding for bacteriochlorophyll synthase, EC 2.5.1.133 Synechocystis sp. PCC 6803

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.B43 F28I naturally occuring mutation, the mutant shows chlorophyll synthase, EC 2.5.1.62, ChlG, activity Cereibacter sphaeroides
2.5.1.B43 additional information a BchG mutant Rhodobacter sphaeroides BG1 strain is photosynthetically incompetent. Expression of gene chlG from Synechocystis sp. PCC 6803, encoding a chlorophyll synthase I44F mutant, EC 2.5.1.62, rescues the photoheterotrophical growth of the mutant cells. The Synechocystis mutated enzyme ChlGI44F shows phytyl-dependent bacteriohchlorophyll a synthase activity Cereibacter sphaeroides
2.5.1.62 I44F naturyll occuring mutant, that also shows bacteriochlorophyl syntase activity, EC 2.5.1.133 Synechocystis sp. PCC 6803

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.B43 0.13
-
 bacteriochlorophyllide a pH 7.6, 30°C, recombinant wild-type enzyme Cereibacter sphaeroides
2.5.1.B43 0.16
-
 bacteriochlorophyllide a pH 7.6, 30°C, recombinant enzyme mutant R28I Cereibacter sphaeroides
2.5.1.62 0.09
-
 chlorophyllide a pH 7.6, 30°C, recombinant wild-type enzyme Synechocystis sp. PCC 6803
2.5.1.62 0.11
-
 chlorophyllide a pH 7.6, 30°C, recombinant enzyme mutant I44F Synechocystis sp. PCC 6803

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.B43 Mg2+ required Cereibacter sphaeroides
2.5.1.62 Mg2+ required Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B43 bacteriochlorophyllide a + phytyl diphosphate Cereibacter sphaeroides
-
 bacteriochlorophyll a + diphosphate
-
 ?
2.5.1.B43 additional information Cereibacter sphaeroides bacteriochlorophyll synthase has a high degree of substrate specificity ?
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate Synechocystis sp. PCC 6803
-
 chlorophyll a + diphosphate
-
 ?
2.5.1.62 additional information Synechocystis sp. PCC 6803 chlorophyll synthase has a high degree of substrate specificity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B43 Cereibacter sphaeroides Q9Z5D6 gene bchG
-
2.5.1.62 Synechocystis sp. PCC 6803 Q55145 gene name chlG
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B43 bacteriochlorophyllide a + phytyl diphosphate
-
 Cereibacter sphaeroides bacteriochlorophyll a + diphosphate
-
 ?
2.5.1.B43 additional information bacteriochlorophyll synthase has a high degree of substrate specificity Cereibacter sphaeroides ?
-
 ?
2.5.1.62 chlorophyllide a + phytyl diphosphate
-
 Synechocystis sp. PCC 6803 chlorophyll a + diphosphate
-
 ?
2.5.1.62 additional information chlorophyll synthase has a high degree of substrate specificity Synechocystis sp. PCC 6803 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B43 bchG
-
 Cereibacter sphaeroides
2.5.1.62 ChlG
-
 Synechocystis sp. PCC 6803

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B43 30
-
 assay at Cereibacter sphaeroides
2.5.1.62 30
-
 assay at Synechocystis sp. PCC 6803

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B43 7.6
-
 assay at Cereibacter sphaeroides
2.5.1.62 7.6
-
 assay at Synechocystis sp. PCC 6803

General Information

EC Number General Information Comment Organism
2.5.1.B43 evolution the predicted sequence of ChlG of Synechocystis sp. PCC 6803 is considerably similar (35% identity) to that of Rhodobacter sphaeroides BchG, each enzyme has a high level of substrate specificity to distinguish its own substrate from the other. But the BchG activity of Synechocystis chlorophyll synthase mutant ChlGI44F and the ChlG activity of bacteriochlorophyll synthase mutant BchGF28I suggest that ChlG/EC 2.5.1.62 and BchG/EC 2.5.1.B43 are evolutionarily related enzymes, cf. EC 2.5.1.133 Cereibacter sphaeroides
2.5.1.B43 additional information residue F28 is important for the substrate specificities of the enzyme Cereibacter sphaeroides
2.5.1.62 evolution the predicted sequence of ChlG of Synechocystis sp. PCC 6803 is considerably similar (35% identity) to that of Rhodobacter sphaeroides BchG, each enzyme has a high level of substrate specificity to distinguish its own substrate from the other. But the BchG activity of Synechocystis chlorophyll synthase mutant ChlGI44F and the ChlG activity of bacteriochlorophyll synthase mutant BchGF28I suggest that ChlG/EC 2.5.1.62 and BchG/EC 2.5.1.133 are evolutionarily related enzymes Synechocystis sp. PCC 6803
2.5.1.62 additional information residue I44 is important for the substrate specificities of ChlG Synechocystis sp. PCC 6803