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Literature summary extracted from

  • Mietla, J.A.; Wijesinghe, D.S.; Hoeferlin, L.A.; Shultz, M.D.; Natarajan, R.; Fowler, A.A.; Chalfant, C.E.
    Characterization of eicosanoid synthesis in a genetic ablation model of ceramide kinase (2013), J. Lipid Res., 54, 1834-1847.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.138 additional information generation of CERK-/- knockout mice and enzyme silencing by siRNA Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.138 Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.138 ATP + ceramide Mus musculus
-
 ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide Mus musculus BALB/c
-
 ADP + ceramide 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.138 Mus musculus Q8K4Q7 gene CERK
-
2.7.1.138 Mus musculus BALB/c Q8K4Q7 gene CERK
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.138 macrophage
-
 Mus musculus
-
2.7.1.138 MEF cell primary mouse embryonic fibroblasts Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.138 ATP + ceramide
-
 Mus musculus ADP + ceramide 1-phosphate
-
 ?
2.7.1.138 ATP + ceramide
-
 Mus musculus BALB/c ADP + ceramide 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.138 CERK
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.138 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.1.138 malfunction genetic ablation of CERK reduces basal ceramide 1-phosphate levels. CERK-/- knockout mouse cells show 52-74% reduced ceramide 1-phosphate contents and significantly lower levels of multiple eicosanoids compared to wild-type. Induction of eicosanoid synthesis by calcium ionophore is significantly reduced in the CERK-/- primary mouse embryonic fibroblasts. The CERK-/- mouse has adapted to loss of CERK in regards to airway hyper-responsiveness as compared with CERK siRNA treatment. Eicosanoids produced by immortalized primary mouse embryonic fibroblasts, overview Mus musculus
2.7.1.138 physiological function a role for ceramide kinase in the production of eicosanoids. The initial rate-limiting step in eicosanoid biosynthesis is started by the activation of group IVA phospholipase A, 2cPLA2alpha, which is activated by direct binding of the C2 domain to ceramide-1-phosphate Mus musculus