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Literature summary extracted from

  • Mejias, M.P.; Ghersi, G.; Craig, P.O.; Panek, C.A.; Bentancor, L.V.; Baschkier, A.; Goldbaum, F.A.; Zylberman, V.; Palermo, M.S.
    Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella lumazine synthase confers total protection against Shiga toxins in mice (2013), J. Immunol., 191, 2403-2411.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.78 medicine the striking feature of enterohemorrhagic Escherichia coli (EHEC) infections is the production of Shiga toxins (Stx) implicated in the development of the life-threatening hemolytic uremic syndrome. Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase confers total protection against Shiga toxins in mice, analysis of antibody efficacy against EHEC challenge. The enzyme can be used as transport vehhicle and antigen in vaccination against EHEC infections Brucella sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.78 recombinant expression of the enzyme in Escherichia coli, and of the enzyme fused to Shiga toxin 2 subunit B, resulting in the BLS-Stx2B chimera, in Escherichia coli strain JM109 Brucella sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.78 additional information construction of a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase, BLS-Stx2B chimera, confers total protection against Shiga toxins in mice after immunization Brucella sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.78 234000
-
 recombinant chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase, i.e. BLS-Stx2B chimera, gel filtration Brucella sp.

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.78 Brucella sp.
-
-
-

Subunits

EC Number Subunits Comment Organism
2.5.1.78 More BLS folds as a decamer in the recombinant BLS-Stx2B chimera, i.e. a chimera consisting of the B subunit of Shiga toxin type 2 and Brucella sp. lumazine synthase Brucella sp.

Synonyms

EC Number Synonyms Comment Organism
2.5.1.78 BLS
-
 Brucella sp.
2.5.1.78 lumazine synthase
-
 Brucella sp.

General Information

EC Number General Information Comment Organism
2.5.1.78 additional information molecular modelling of the recombinant BLS-Stx2B chimera fusing the C-terminal end of the structure of Stx2B, PDB ID IR4P, with the N-terminus of the crystallographic structure of enzyme BLS, PDB ID IDI0, through a decapeptide linker of sequence GSGSGSGSGS Brucella sp.