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Literature summary extracted from
- Two glycerol 3-phosphate dehydrogenase isogenes from Candida versatilis SN-18 play an important role in glycerol biosynthesis under osmotic stress (2016), J. Biosci. Bioeng., 121, 523-529.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.8 | expressed in a gpd1DELTAgpd2DELTA double deletion mutant of Saccharomyces cerevisiae | Wickerhamiella versatilis |
| 1.1.1.8 | expressed in Saccharomyces cerevisiae strain BY4741 | Wickerhamiella versatilis |
| 1.1.1.8 | gene Cagpd1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant | Wickerhamiella versatilis |
| 1.1.1.8 | gene Cagpd2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval. Recombinant expression in the Sacchhyromyces cerevisiae strain BY4741 gpd1DELTAgpd2DELTA double mutant | Wickerhamiella versatilis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis | - |
glycerone phosphate + NADH + H+ | - |
? |  |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis | - |
glycerone phosphate + NADH + H+ | - |
r | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis SN-18 | - |
glycerone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | Wickerhamiella versatilis SN-18 | - |
glycerone phosphate + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.8 | Wickerhamiella versatilis | A0A140JW76 | - |
- |
| 1.1.1.8 | Wickerhamiella versatilis | A0A140JW76 | an osmotolerant yeast isolated from soil outside a food factory | - |
| 1.1.1.8 | Wickerhamiella versatilis | A0A140JW77 | - |
- |
| 1.1.1.8 | Wickerhamiella versatilis | A0A140JW77 | an osmotolerant yeast isolated from soil outside a food factory | - |
| 1.1.1.8 | Wickerhamiella versatilis SN-18 | A0A140JW76 | - |
- |
| 1.1.1.8 | Wickerhamiella versatilis SN-18 | A0A140JW76 | an osmotolerant yeast isolated from soil outside a food factory | - |
| 1.1.1.8 | Wickerhamiella versatilis SN-18 | A0A140JW77 | - |
- |
| 1.1.1.8 | Wickerhamiella versatilis SN-18 | A0A140JW77 | an osmotolerant yeast isolated from soil outside a food factory | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 0.0009 | - |
crude enzyme extract, pH 8.5, 30°C | Wickerhamiella versatilis |
| 1.1.1.8 | 0.00147 | - |
crude enzyme extract, pH 8.5, 30°C | Wickerhamiella versatilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.8 | additional information | no activity with NADP+ | Wickerhamiella versatilis | ? | - |
? | |
| 1.1.1.8 | additional information | no activity with NADP+ | Wickerhamiella versatilis SN-18 | ? | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis | glycerone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis | glycerone phosphate + NADH + H+ | - |
r | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis SN-18 | glycerone phosphate + NADH + H+ | - |
? | |
| 1.1.1.8 | sn-glycerol 3-phosphate + NAD+ | - |
Wickerhamiella versatilis SN-18 | glycerone phosphate + NADH + H+ | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.8 | Cagpd1 | - |
Wickerhamiella versatilis |
| 1.1.1.8 | Cagpd1 | isoform | Wickerhamiella versatilis |
| 1.1.1.8 | Cagpd1p | - |
Wickerhamiella versatilis |
| 1.1.1.8 | Cagpd2 | - |
Wickerhamiella versatilis |
| 1.1.1.8 | Cagpd2 | isoform | Wickerhamiella versatilis |
| 1.1.1.8 | Cagpd2p | - |
Wickerhamiella versatilis |
| 1.1.1.8 | glycerol 3-phosphate dehydrogenase | - |
Wickerhamiella versatilis |
| 1.1.1.8 | GPD | - |
Wickerhamiella versatilis |
| 1.1.1.8 | Gpd1p | - |
Wickerhamiella versatilis |
| 1.1.1.8 | gpd2p | - |
Wickerhamiella versatilis |
| 1.1.1.8 | NADP+-dependent glycerol 3-phosphate dehydrogenase | - |
Wickerhamiella versatilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 30 | - |
assay at | Wickerhamiella versatilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.8 | 8.5 | - |
assay at | Wickerhamiella versatilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.8 | additional information | Cagpd1p is a NAD+-dependent GPD, no activity with NADP+ | Wickerhamiella versatilis | |
| 1.1.1.8 | additional information | Cagpd2p is a NAD+-dependent GPD, no activity with NADP+ | Wickerhamiella versatilis | |
| 1.1.1.8 | NAD+ | - |
Wickerhamiella versatilis | |
| 1.1.1.8 | NADH | - |
Wickerhamiella versatilis | |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.1.1.8 | Wickerhamiella versatilis | osmotic stress induces the isozyme | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.8 | evolution | the intronless genes Cagpd1 and Cagpd2, encoding the two isozymes of the organism, are both predicted to encode a 378 amino acid polypeptide, and the deduced amino acid sequences mutually show 76% identity. Genes Cagpd1 and Cagpd2 are located tandemly in a locus of genomic DNA within a 262 bp interval | Wickerhamiella versatilis |
| 1.1.1.8 | metabolism | the enzyme is a key enzyme in the glycerol biosynthesis pathway | Wickerhamiella versatilis |
| 1.1.1.8 | physiological function | the enzyme is involved in osmotic stress response | Wickerhamiella versatilis |
| 1.1.1.8 | physiological function | two glycerol 3-phosphate dehydrogenase isozymes from Candida versatilis SN-18 play an important role in glycerol biosynthesis under osmotic stress. The enzyme is involved in osmotic stress response | Wickerhamiella versatilis |
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Release 2023.1 (January 2023)
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