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Literature summary extracted from

  • Ramachandran, A.; Nandakumar, D.; Deshpande, A.P.; Lucas, T.P.; R-Bhojappa, R.; Tang, G.Q.; Raney, K.; Yin, Y.W.; Patel, S.S.
    The yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA (2016), J. Biol. Chem., 291, 16828-16839.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.7.6 molecular biology Rpo41-Mtf1 is an attractive candidate for serving as the primase to initiate lagging strand DNA synthesis during normal replication and/or to restart stalled replication from downstream ssDNA Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 protein Rim1 the ssDNA-binding protein Rim1 severely inhibits theRNAsynthesis activity of Rpo41, but not the Rpo41-Mtf1 complex, which continues to prime DNA synthesis efficiently in the presence of Rim1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.6 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 additional information Saccharomyces cerevisiae mitochondrial RNA polymerase (Rpo41) and its transcription factor (Mtf1) are an efficient primase that initiates DNA synthesis on ssDNA coated with the yeast mitochondrial ssDNA-binding protein, Rim1. Both Rpo41 and Rpo41-Mtf1 can synthesize short and long RNAs on ssDNA template and prime DNA synthesis by the yeast mitochondrial DNA polymerase Mip1. Regarding the RNA-DNA products, Rpo41 and Rpo41-Mtf1 have slightly different priming specificity. Both prefer to initiate with ATP from short priming sequences such as 3'-TCC, TTC, and TTT, and the consensus sequence is 3'-Pu(Py)2-3 ?
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Saccharomyces cerevisiae
-
 diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 additional information mitochondrial RNA polymerase (Rpo41) and its transcription factor (Mtf1) are an efficient primase that initiates DNA synthesis on ssDNA coated with the yeast mitochondrial ssDNA-binding protein, Rim1. Both Rpo41 and Rpo41-Mtf1 can synthesize short and long RNAs on ssDNA template and prime DNA synthesis by the yeast mitochondrial DNA polymerase Mip1. Regarding the RNA-DNA products, Rpo41 and Rpo41-Mtf1 have slightly different priming specificity. Both prefer to initiate with ATP from short priming sequences such as 3'-TCC, TTC, and TTT, and the consensus sequence is 3'-Pu(Py)2-3 Saccharomyces cerevisiae ?
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn
-
 Saccharomyces cerevisiae diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn Rpo41 and Rpo41-Mtf1 synthesize RNA on M13 ssDNA template Saccharomyces cerevisiae diphosphate + RNAn+1 30-nt and 41-nt products of Rpo41 and 30-nt, 41-nt, and 60-nt products of Rpo41-Mtf1 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 mitochondrial RNA polymerase
-
 Saccharomyces cerevisiae
2.7.7.6 Rpo41
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.6 30
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.7.7.6 physiological function the yeast mitochondrial RNA polymerase and transcription factor complex catalyzes efficient priming of DNA synthesis on single-stranded DNA. Primases are specialized DNA-dependent RNA polymerases that synthesize short oligoribonucleotides de novo on single-stranded (ss) DNA templates Saccharomyces cerevisiae