Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lee, S.G.; Nwumeh, R.; Jez, J.M.
    Structure and mechanism of isopropylmalate dehydrogenase from Arabidopsis thaliana: insights on leucine and aliphatic glucosinolate biosynthesis (2016), J. Biol. Chem., 291, 13421-13430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.85 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.85 in complex with either isopropylmalate and Mg2+ or NAD+, hanging drop vapor diffusion method, using 1.0 M ammonium phosphate, 0.1 M imidazole, pH 8.0 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.85 D264N inactive Arabidopsis thaliana
1.1.1.85 D288N inactive Arabidopsis thaliana
1.1.1.85 D292N the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 K232M inactive Arabidopsis thaliana
1.1.1.85 L132A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 L133A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 N234A inactive Arabidopsis thaliana
1.1.1.85 N234D inactive Arabidopsis thaliana
1.1.1.85 R136A inactive Arabidopsis thaliana
1.1.1.85 R136K the mutant shows very strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 R146A the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 R146K the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 R174A inactive Arabidopsis thaliana
1.1.1.85 R174K the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 V235A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 Y181A the mutant shows strongly reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 Y181F the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana
1.1.1.85 Y181H the mutant shows reduced catalytic efficiency compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.85 0.0035
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0063
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181H, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0074
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181F, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0083
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0114
-
 (2R,3S)-3-isopropylmalate mutant enzyme V235A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0242
-
 (2R,3S)-3-isopropylmalate mutant enzyme L132A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0278
-
 (2R,3S)-3-isopropylmalate mutant enzyme D292N, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.0707
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.119
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.125
-
 (2R,3S)-3-isopropylmalate mutant enzyme L133A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.753
-
 (2R,3S)-3-isopropylmalate mutant enzyme R174K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 3.73
-
 (2R,3S)-3-isopropylmalate mutant enzyme R136K, at pH 7.5 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+ Arabidopsis thaliana
-
 4-methyl-2-oxovalerate + CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.85 Arabidopsis thaliana P93882
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.85 Ni-NTA column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.85 (2R,3S)-3-isopropylmalate + NAD+
-
 Arabidopsis thaliana 4-methyl-2-oxovalerate + CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.85 IPMDH2
-
 Arabidopsis thaliana
1.1.1.85 isopropylmalate dehydrogenase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.85 0.003
-
 (2R,3S)-3-isopropylmalate mutant enzyme D292N, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.017
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.023
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.03
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.033
-
 (2R,3S)-3-isopropylmalate mutant enzyme R136K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.233
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181F, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.47
-
 (2R,3S)-3-isopropylmalate mutant enzyme L132A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.583
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181H, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 1.62
-
 (2R,3S)-3-isopropylmalate mutant enzyme R174K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 2
-
 (2R,3S)-3-isopropylmalate mutant enzyme V235A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 3.1
-
 (2R,3S)-3-isopropylmalate mutant enzyme L133A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 4.57
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.85 NAD+
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.85 0.009
-
 (2R,3S)-3-isopropylmalate mutant enzyme R136K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.109
-
 (2R,3S)-3-isopropylmalate mutant enzyme D292N, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.192
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 0.429
-
 (2R,3S)-3-isopropylmalate mutant enzyme R146A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 1.92
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 2.14
-
 (2R,3S)-3-isopropylmalate mutant enzyme R174K, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 19.29
-
 (2R,3S)-3-isopropylmalate mutant enzyme L132A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 24.83
-
 (2R,3S)-3-isopropylmalate mutant enzyme L133A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 32.32
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181F, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 91.21
-
 (2R,3S)-3-isopropylmalate mutant enzyme Y181H, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 180.3
-
 (2R,3S)-3-isopropylmalate mutant enzyme V235A, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.85 1303
-
 (2R,3S)-3-isopropylmalate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana