Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lange, C.; Kiesel, S.; Peters, S.; Virus, S.; Scheer, H.; Jahn, D.; Moser, J.
    Broadened substrate specificity of 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase (BchC) indicates a new route for the biosynthesis of bacteriochlorophyll a (2015), J. Biol. Chem., 290, 19697-19709.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.396 expressed in Escherichia coli BL21(DE3) cells Chlorobaculum tepidum
4.2.1.165 expression in Escherichia coli Chlorobaculum tepidum

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.396 E46A inactive Chlorobaculum tepidum
1.1.1.396 E68A inactive Chlorobaculum tepidum
1.1.1.396 H141A inactive Chlorobaculum tepidum
1.1.1.396 Y67A inactive Chlorobaculum tepidum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.165 membrane integral membrane protein, four putative membrane-spanning helices comprising the transmembrane segments Ile23-Tyr42,Thr56-Glu77, Phe94-Gln113, and His118-Gln140 Chlorobaculum tepidum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.396 Zn2+ required Chlorobaculum tepidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.396 37000
-
 x * 37000, SDS-PAGE Chlorobaculum tepidum
4.2.1.165 25000
-
-
 Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.396 3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+ Chlorobaculum tepidum
-
 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+ Chlorobaculum tepidum TLS
-
 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-hydroxyethyl chlorophyllide a + NAD+ Chlorobaculum tepidum
-
 bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-hydroxyethyl chlorophyllide a + NAD+ Chlorobaculum tepidum TLS
-
 bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 bacteriopheophorbide a + NAD+ Chlorobaculum tepidum
-
 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 bacteriopheophorbide a + NAD+ Chlorobaculum tepidum TLS
-
 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 additional information Chlorobaculum tepidum no activity with 3-hydroxyethyl-bacteriochlorophyll a ?
-
 ?
1.1.1.396 additional information Chlorobaculum tepidum TLS no activity with 3-hydroxyethyl-bacteriochlorophyll a ?
-
 ?
4.2.1.165 3-vinyl-bacteriochlorophyllide a + H2O Chlorobaculum tepidum
-
 3-devinyl-3-(1-hydroxyethyl)-bacteriochlorophyllide a
-
 ?
4.2.1.165 3-vinyl-bacteriochlorophyllide a + H2O Chlorobaculum tepidum DSM 12025
-
 3-devinyl-3-(1-hydroxyethyl)-bacteriochlorophyllide a
-
 ?
4.2.1.165 chlorophyllide a + H2O Chlorobaculum tepidum
-
 3-devinyl-3-(1-hydroxyethyl)-chlorophyllide a
-
 ?
4.2.1.165 chlorophyllide a + H2O Chlorobaculum tepidum DSM 12025
-
 3-devinyl-3-(1-hydroxyethyl)-chlorophyllide a
-
 ?
4.2.1.165 additional information Chlorobaculum tepidum in bacteriochlorophyll a biosynthesis, 3-vinyl bacteriochlorophyllide a hydratase BchF converts the 3-hydroxyethyl group on pyrrole ring A into a 3-acetyl group ?
-
 ?
4.2.1.165 additional information Chlorobaculum tepidum DSM 12025 in bacteriochlorophyll a biosynthesis, 3-vinyl bacteriochlorophyllide a hydratase BchF converts the 3-hydroxyethyl group on pyrrole ring A into a 3-acetyl group ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.396 Chlorobaculum tepidum Q8KCJ7
-
-
1.1.1.396 Chlorobaculum tepidum TLS Q8KCJ7
-
-
4.2.1.165 Chlorobaculum tepidum H2VFK0
-
-
4.2.1.165 Chlorobaculum tepidum DSM 12025 H2VFK0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.396 glutathione-agarose column chromatography, and gel filtration Chlorobaculum tepidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.396 3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+
-
 Chlorobaculum tepidum 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-(hydroxy)ethyl-13-hydroxy-pheophorbide a + NAD+
-
 Chlorobaculum tepidum TLS 3-acetyl-13-hydroxy-pheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-hydroxyethyl chlorophyllide a + NAD+
-
 Chlorobaculum tepidum bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 3-hydroxyethyl chlorophyllide a + NAD+
-
 Chlorobaculum tepidum TLS bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 bacteriopheophorbide a + NAD+
-
 Chlorobaculum tepidum 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 bacteriopheophorbide a + NAD+
-
 Chlorobaculum tepidum TLS 3-hydroxyethyl-bacteriopheophorbide a + NADH + H+
-
 ?
1.1.1.396 additional information no activity with 3-hydroxyethyl-bacteriochlorophyll a Chlorobaculum tepidum ?
-
 ?
1.1.1.396 additional information no activity with 3-hydroxyethyl-bacteriochlorophyll a Chlorobaculum tepidum TLS ?
-
 ?
4.2.1.165 3-vinyl-bacteriochlorophyllide a + H2O
-
 Chlorobaculum tepidum 3-devinyl-3-(1-hydroxyethyl)-bacteriochlorophyllide a
-
 ?
4.2.1.165 3-vinyl-bacteriochlorophyllide a + H2O
-
 Chlorobaculum tepidum DSM 12025 3-devinyl-3-(1-hydroxyethyl)-bacteriochlorophyllide a
-
 ?
4.2.1.165 chlorophyllide a + H2O
-
 Chlorobaculum tepidum 3-devinyl-3-(1-hydroxyethyl)-chlorophyllide a
-
 ?
4.2.1.165 chlorophyllide a + H2O
-
 Chlorobaculum tepidum DSM 12025 3-devinyl-3-(1-hydroxyethyl)-chlorophyllide a
-
 ?
4.2.1.165 additional information in bacteriochlorophyll a biosynthesis, 3-vinyl bacteriochlorophyllide a hydratase BchF converts the 3-hydroxyethyl group on pyrrole ring A into a 3-acetyl group Chlorobaculum tepidum ?
-
 ?
4.2.1.165 additional information in bacteriochlorophyll a biosynthesis, 3-vinyl bacteriochlorophyllide a hydratase BchF converts the 3-hydroxyethyl group on pyrrole ring A into a 3-acetyl group Chlorobaculum tepidum DSM 12025 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.396 ? x * 37000, SDS-PAGE Chlorobaculum tepidum
1.1.1.396 ? x * 37068, calculated from amino acid sequence Chlorobaculum tepidum
4.2.1.165 ? x * 25000, SDS-PAGE, 20008, calculated Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.396 3-hydroxyethyl bacteriochlorophyllide a dehydrogenase
-
 Chlorobaculum tepidum
1.1.1.396 bchC
-
 Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.396 NAD+
-
 Chlorobaculum tepidum