EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.15.28 | in complex with cholesteryl sulfate, sitting drop vapor diffusion method, using 20-25% PEG 3350, 10-13% (v/v) glycerol, and 50 mM MgCl2 | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.15.28 | 0.0398 | - |
cholest-4-en-3-one | at pH 7.4 and 25°C | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | Mycolicibacterium smegmatis | - |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.28 | Mycolicibacterium smegmatis | A0R4Q6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.28 | nickel-nitrilotriacetic acid column chromatography and Q-Sepharose column chromatography | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin | - |
Mycolicibacterium smegmatis | (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.28 | CYP142A2 | - |
Mycolicibacterium smegmatis |