BRENDA - Enzyme Database

Cholesterol ester oxidation by mycobacterial cytochrome P450

Frank, D.J.; Madrona, Y.; Ortiz de Montellano, P.R.; J. Biol. Chem. 289, 30417-30425 (2014)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.14.15.28
in complex with cholesteryl sulfate, sitting drop vapor diffusion method, using 20-25% PEG 3350, 10-13% (v/v) glycerol, and 50 mM MgCl2
Mycolicibacterium smegmatis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.28
0.0398
-
cholest-4-en-3-one
at pH 7.4 and 25°C
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.28
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.28
Mycolicibacterium smegmatis
A0R4Q6
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.28
nickel-nitrilotriacetic acid column chromatography and Q-Sepharose column chromatography
Mycolicibacterium smegmatis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.28
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738629
Mycolicibacterium smegmatis
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.14.15.28
in complex with cholesteryl sulfate, sitting drop vapor diffusion method, using 20-25% PEG 3350, 10-13% (v/v) glycerol, and 50 mM MgCl2
Mycolicibacterium smegmatis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.28
0.0398
-
cholest-4-en-3-one
at pH 7.4 and 25°C
Mycolicibacterium smegmatis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.15.28
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
Mycolicibacterium smegmatis
-
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.28
nickel-nitrilotriacetic acid column chromatography and Q-Sepharose column chromatography
Mycolicibacterium smegmatis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.28
cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
738629
Mycolicibacterium smegmatis
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
-
-
?