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Literature summary extracted from

  • Liu, J.; Ryabichko, S.; Bogdanov, M.; Fackelmayer, O.J.; Dowhan, W.; Krulwich, T.A.
    Cardiolipin is dispensable for oxidative phosphorylation and non-fermentative growth of alkaliphilic Bacillus pseudofirmus OF4 (2014), J. Biol. Chem., 289, 2960-2971.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.B10 three putative Cl synthase encoding genes: clsA, clsB, and clsC Alkalihalophilus pseudofirmus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.B10 additional information construction of three single, DELTAclsA, DELTAclsB, and DELTAclsC, one double, DELTAclsA/B, and one triple, DELTAclsA/B/C, mutants Alkalihalophilus pseudofirmus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.B10 membrane
-
Alkalihalophilus pseudofirmus 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.B10 Alkalihalophilus pseudofirmus O66043 and methionine auxotroph derivative strain 811M
-
2.7.8.B10 Alkalihalophilus pseudofirmus OF4 O66043 and methionine auxotroph derivative strain 811M
-

Synonyms

EC Number Synonyms Comment Organism
2.7.8.B10 CL synthase
-
Alkalihalophilus pseudofirmus
2.7.8.B10 CLS
-
Alkalihalophilus pseudofirmus
2.7.8.B10 clsA
-
Alkalihalophilus pseudofirmus
2.7.8.B10 clsB
-
Alkalihalophilus pseudofirmus
2.7.8.B10 clsC
-
Alkalihalophilus pseudofirmus

General Information

EC Number General Information Comment Organism
2.7.8.B10 malfunction no detectablecardiolipin is found in DELTAclsA mutants, whereas the cardiolipin precursor phosphatidylglycerol is elevated. The DELTAclsB mutant exhibits no significant reduction in cardiolipin, but at pH 7.5, clsB expression is upregulated and appears growth. In the absence of detectable cardiolipin, the alkaliphile shows no significant deficits in non-fermentative growth, respiration-dependent ATP synthesis, or salt tolerance. In long term survival experiments, significant growth defects are found in DELTAclsA strains and the DELTAclsC strain at pH10.5. Both the single DELTAclsA and triple deletion mutants have a significant reduction in NADH dehydrogenase activity Alkalihalophilus pseudofirmus
2.7.8.B10 metabolism the clsA gene plays a major role in cardiolipin biosynthesis Alkalihalophilus pseudofirmus
2.7.8.B10 physiological function cardiolipin is not essential for oxidative phosphorylation and the non-fermentative growth it supports in alkaliphilic Bacillus pseudofirmus strain OF4, during growth at either pH 7.5 or pH 10.5. Cardiolipin does not seem be a crucial participant in the proton movements on or near the membrane surface from proton-pumping respiratory chain complexes to the F1F0-ATP synthase complex Alkalihalophilus pseudofirmus