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Literature summary extracted from

  • Das, U.; Wang, L.K.; Smith, P.; Munir, A.; Shuman, S.
    Structures of bacterial polynucleotide kinase in a Michaelis complex with nucleoside triphosphate (NTP)-Mg2+ and 5-OH RNA and a mixed substrate-product complex with NTP-Mg2+ and a 5-phosphorylated oligonucleotide (2014), J. Bacteriol., 196, 4285-4292.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.78 expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.78 mutant enzyme D38N in complex with GTP-Mg2+ and a 5'-OH RNA oligonucleotide, hanging drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 100 mM MgCl2, 18-24% (w/v) PEG-6000 Acetivibrio thermocellus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.78 Q51A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus
2.7.1.78 S37A/T80A the double mutation reduces kinase activity 50fold Acetivibrio thermocellus
2.7.1.78 T54A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus
2.7.1.78 V129A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Mg2+ required Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Acetivibrio thermocellus the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Acetivibrio thermocellus DSM 1237 the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Acetivibrio thermocellus A3DJ38
-
-
2.7.1.78 Acetivibrio thermocellus DSM 1237 A3DJ38
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 Ni-agarose column chromatography and Superdex 200 gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 Acetivibrio thermocellus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 Acetivibrio thermocellus DSM 1237 ADP + 5'-phospho-DNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 homodimer x-ray crystallography Acetivibrio thermocellus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 PNK
-
 Acetivibrio thermocellus
2.7.1.78 polynucleotide kinase
-
 Acetivibrio thermocellus