Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sobota, J.M.; Gu, M.; Imlay, J.A.
    Intracellular hydrogen peroxide and superoxide poison 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, the first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli (2014), J. Bacteriol., 196, 1980-1991.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.54 gene aroG, recombinant expression of His10-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.54 H2O2 DAHP synthase enzymes are inactivated by H2O2 in vitro and in vivo, H2O2 displaces the iron atom from the enzyme, only the Fe2+-metalloform of the enzyme can be inactivated by hydrogen peroxide or superoxide Escherichia coli
2.5.1.54 additional information isozyme AroG is not inhibited by L-Phe. In peroxide-stressed cells, the enzyme accumulates as an apoprotein, potentially with an oxidized cysteine residue. In superoxide-stressed cells, the enzyme acquires a nonactivating zinc ion in its active site, an apparent consequence of the repeated ejection of iron. Manganese supplementation protects the activity in both cases, which matches the ability of manganese to metallate the enzyme and to provide substantial oxidant-resistant activity. The damage to DAHP synthase can be completely restored in vivo, while in vitro, restoration is only partly, overview. Escherichia coli attempts to compensate for diminished DAHP synthase activity by increasing expression Escherichia coli
2.5.1.54 superoxide displaces the iron atom from the enzyme, only the Fe2+-metalloform of the enzyme can be inactivated by hydrogen peroxide or superoxide. Superoxide stress promotes the mismetallation of DAHP synthase Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.54 Fe2+ a mononuclear iron enzyme, the enzyme required divalent metal cations, Fe2+ activates best, iron is the prosthetic metal in vivo Escherichia coli
2.5.1.54 Mn2+ can substitute for Fe2+, activates DAHP synthase to about 70% of the iron-activated sample Escherichia coli
2.5.1.54 Zn2+ cannot well substitute for Fe2+, activates DAHP synthase to about 20% of the iron-activated sample Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O Escherichia coli
-
 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.54 Escherichia coli P0AB91 gene aroG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.54 recombinant His10-tagged enzyme from Escherichia coli strain BL21 by affinity chromatography, followed by tag cleavage with factor Xa and again affinity chromatography and dialysis to remove the tag Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.54 phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Escherichia coli 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.54 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase
-
 Escherichia coli
2.5.1.54 DAHP synthase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.54 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.54 6.4
-
 assay at Escherichia coli

Expression

EC Number Organism Comment Expression
2.5.1.54 Escherichia coli enzyme damage by reactive oxidants, H2O2 and superoxide, induces the enzyme expression in Escherichia coli, which attempts to compensate for diminished DAHP synthase activity by increasing expression up

General Information

EC Number General Information Comment Organism
2.5.1.54 evolution DAHP synthase belongs to a family of mononuclear iron-containing enzymes that are disabled by oxidative stress Escherichia coli
2.5.1.54 metabolism first committed enzyme in the aromatic biosynthetic pathway of Escherichia coli, shikimate biosynthesis pathway overview Escherichia coli