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Literature summary extracted from

  • Yang, Y.; Zhang, L.; Guo, M.; Sun, J.; Matsukawa, S.; Xie, J.; Wei, D.
    Novel alpha-L-arabinofuranosidase from Cellulomonas fimi ATCC 484 and its substrate-specificity analysis with the aid of computer (2015), J. Agric. Food Chem., 63, 3725-3733.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.58 molecular modeling of structure. Residue Glu173 is the acid/base, and Glu292 is the nucleophile Cellulomonas fimi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 1.5
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C Cellulomonas fimi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.58 55600
-
-
 Cellulomonas fimi
3.2.1.58 66000
-
-
 Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Cellulomonas fimi F4H166
-
-
3.2.1.58 Cellulomonas fimi ATCC 484 F4H166
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.58 19.4
-
 substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 6.0, 40°C Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 4-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Cellulomonas fimi 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.58 4-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Cellulomonas fimi ATCC 484 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.58 linear alpha-1,5-L-arabinan + H2O
-
 Cellulomonas fimi ?
-
 ?
3.2.1.58 linear alpha-1,5-L-arabinan + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?
3.2.1.58 additional information no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside Cellulomonas fimi ?
-
 ?
3.2.1.58 additional information no substrates: beechwood xylan, soluble starch, gum arabic, and larch arabinogalactan, 4-nitrophenyl alpha-D-xylopyranoside and 4-nitrophenyl beta-D-xylopyranoside Cellulomonas fimi ATCC 484 ?
-
 ?
3.2.1.58 sugar beet arabinan + H2O
-
 Cellulomonas fimi ?
-
 ?
3.2.1.58 sugar beet arabinan + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?
3.2.1.58 wheat flour arabinoxylan + H2O
-
 Cellulomonas fimi ?
-
 ?
3.2.1.58 wheat flour arabinoxylan + H2O
-
 Cellulomonas fimi ATCC 484 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.58 ? x * 55600, calculated, x * 66000, SDS-PAGE Cellulomonas fimi

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 Celf_3321
-
 Cellulomonas fimi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.58 123.6
-
 4-nitrophenyl-alpha-L-arabinofuranoside pH 6.0, 40°C Cellulomonas fimi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 6
-
-
 Cellulomonas fimi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.58 5
-
 58% of maximum activity Cellulomonas fimi
3.2.1.58 7
-
 72% of maximum activity Cellulomonas fimi

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.58 4
-
 4 h, complete loss of activity Cellulomonas fimi
3.2.1.58 6
-
 stable Cellulomonas fimi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.58 Cellulomonas fimi calculated
-
 4.9