EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.73 | homology modeling of structure. The enzyme is primarily composed of a beta-sheet, with two juxtaposed curved antiparallel beta-sheets consisting of seven strands. It forms a single compact globular domain, creating a deep cavity as a carbohydrate-binding channel for beta-glucan. The carbohydrate-binding region is a type B domain | Bacillus sp. (in: Bacteria) |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.73 | K48A | 123% of wild-type activity, thermostability and halostability are enhanced. Catalytic efficiency of mutants is enhanced as a result of the increase in substrate affinity. The half-lives of the mutant increases up to about 7fold at 60-70°C | Bacillus sp. (in: Bacteria) |
3.2.1.73 | K48L | 137% of wild-type activity, thermostability and halostability are enhanced. Catalytic efficiency of mutants is enhanced as a result of the increase in substrate affinity. The half-lives of the mutant increases up to about 7fold at 60-70°C | Bacillus sp. (in: Bacteria) |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.73 | NaCl | 30%, halflife of wild-type 230 h, mutant K48A 694 h, mutant K48L 693 h, respectively | Bacillus sp. (in: Bacteria) |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.73 | additional information | - |
Barley beta-glucan | wild-type, 2.1 mg/ml, mutant K48A 1.6 mg/ml, mutant K48L 1.4 mg/ml, respectively, pH 6.0, 50°C | Bacillus sp. (in: Bacteria) |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.73 | Bacillus sp. (in: Bacteria) | - |
- |
- |
3.2.1.73 | Bacillus sp. (in: Bacteria) KCCM 90078 | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.73 | 82.3 | - |
wild-type, pH 6.0, 50°C | Bacillus sp. (in: Bacteria) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.73 | barley beta-glucan + H2O | - |
Bacillus sp. (in: Bacteria) | beta-glucan oligosaccharides | - |
? | |
3.2.1.73 | barley beta-glucan + H2O | - |
Bacillus sp. (in: Bacteria) KCCM 90078 | beta-glucan oligosaccharides | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.73 | Bg1314 | - |
Bacillus sp. (in: Bacteria) |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.73 | 7.5 | - |
Barley beta-glucan | wild-type, pH 6.0, 50°C | Bacillus sp. (in: Bacteria) | |
3.2.1.73 | 7.5 | - |
Barley beta-glucan | mutant K48A, pH 6.0, 50°C | Bacillus sp. (in: Bacteria) | |
3.2.1.73 | 7.5 | - |
Barley beta-glucan | mutant K48L, pH 6.0, 50°C | Bacillus sp. (in: Bacteria) |