EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.29 | DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3) | Choristoneura fumiferana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | (2E,6E)-farnesyl diphosphate | the enzyme shows substrate inhibition by its FPP substrate at low IPP concentration, suggesting the existence of a mechanism that may regulate intracellular FPP pools | Choristoneura fumiferana | |
2.5.1.29 | geranylgeranyl diphosphate | the enzyme shows product inhibition | Choristoneura fumiferana | |
2.5.1.29 | Mn2+ | required, activates up to 5 mM, inhibitory above 5 mM | Choristoneura fumiferana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | additional information | - |
additional information | Michaelis-Menten kinetics | Choristoneura fumiferana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.29 | Mg2+ | required, activates | Choristoneura fumiferana | |
2.5.1.29 | Mn2+ | required, activates up to 5 mM, inhibitory above 5 mM | Choristoneura fumiferana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | Choristoneura fumiferana | - |
diphosphate + geranylgeranyl diphosphate | - |
? | |
2.5.1.29 | additional information | Choristoneura fumiferana | enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate a C20 prenyl chain | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.29 | Choristoneura fumiferana | U3RD44 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.29 | recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by metal affinity chromatography | Choristoneura fumiferana |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate | the condensation of isopentenyl diphosphate to (2E,6E)-farnesyl diphosphate by GGPPS is a bi-substrate/biproduct (bi-bi) reaction, where the two products are geranylgeranyl diphosphate and diphosphate, which is cleaved from (2E,6E)-farnesyl diphosphate. The inhibitory pattern observed by geranylgeranyl diphosphate indicates a compulsory ordered mechanism for isopentenyl diphosphate and (2E,6E)-farnesyl diphosphate binding the enzyme from Choristoneura fumiferana | Choristoneura fumiferana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | - |
Choristoneura fumiferana | diphosphate + geranylgeranyl diphosphate | - |
? | |
2.5.1.29 | (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate | preferred reaction | Choristoneura fumiferana | diphosphate + geranylgeranyl diphosphate | - |
? | |
2.5.1.29 | dimethylallyl diphosphate + 2 isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.92 | Choristoneura fumiferana | 2 diphosphate + (2Z,6Z)-farnesyl diphosphate | - |
? | |
2.5.1.29 | geranyl diphosphate + isopentenyl diphosphate | the enzyme also performs the synthesis of farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate, reaction of EC 2.5.1.10. The enzyme prefers farnesyl diphosphate over other allylic substrates for coupling with IPP, it behaves like a type-III geranylgeranyl diphosphate synthase. Ligand binding strutcure, overview | Choristoneura fumiferana | ? | - |
? | |
2.5.1.29 | additional information | enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate a C20 prenyl chain | Choristoneura fumiferana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.29 | GGPPS | - |
Choristoneura fumiferana |
2.5.1.29 | type-III GGPPS | - |
Choristoneura fumiferana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 37 | - |
- |
Choristoneura fumiferana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.29 | 7 | - |
- |
Choristoneura fumiferana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.29 | 0.0165 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 37°C, recombinant enzyme | Choristoneura fumiferana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.29 | additional information | molecular homology modeling with CfGGPPS as model sequence and PDB entries 2E8V and 2Q80 (yeast and human GGPPSs) as templates, ligand binding analysis, overview | Choristoneura fumiferana |
2.5.1.29 | physiological function | enzyme geranylgeranyl diphosphate synthase catalyzes the condensation of the non-allylic diphosphate, isopentenyl diphosphate, with allylic diphosphates to generate the C20 prenyl chain used for protein prenylation and diterpenoid biosynthesis | Choristoneura fumiferana |