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Literature summary extracted from

  • Liu, Y.; Chen, B.; Wang, Z.; Liu, L.; Tan, T.
    Functional characterization of a thermostable methionine adenosyltransferase from Thermus thermophilus HB27 (2016), Front. Chem. Sci. Engin., 10, 238-244.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.6 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.6 Cu2+
-
 Thermus thermophilus
2.5.1.6 Fe2+
-
 Thermus thermophilus
2.5.1.6 Mn
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.6 1.2
-
 L-methionine at pH 8.0 and 70°C Thermus thermophilus
2.5.1.6 4.19
-
 ATP at pH 8.0 and 70°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.6 Co2+ high activity in the presence of 20 mM Zn2+ Thermus thermophilus
2.5.1.6 Zn2+ highest activity in the presence of 20 mM Zn2+ Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.6 45000
-
 x * 45000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O Thermus thermophilus
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
2.5.1.6 ATP + L-methionine + H2O Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.6 Thermus thermophilus Q72I53
-
-
2.5.1.6 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72I53
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.6 Ni-NTA column chromatography Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.6 ATP + L-methionine + H2O
-
 Thermus thermophilus phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
2.5.1.6 ATP + L-methionine + H2O
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.6 ? x * 45000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.6 MAT
-
 Thermus thermophilus
2.5.1.6 methionine adenosyltransferase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.6 80
-
-
 Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.6 30 90 the enzyme shows activity at a broad temperature range, from 30 to 90°C. The reaction rate increases significantly with temperature, and reaches maximum at 80°C Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.6 70 90 the enzyme retains 60% of its activity after incubation at 70°C for 24 h. The enzyme is nearly inactivated after 1 h and 10 h incubation at 90°C and 80°C, respectively Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.6 8
-
-
 Thermus thermophilus