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Literature summary extracted from

  • Abarca-Lagunas, M.J.; Rivas-Pardo, J.A.; Ramirez-Sarmiento, C.A.; Guixe, V.
    Dissecting the functional roles of the conserved NXXE and HXE motifs of the ADP-dependent glucokinase from Thermococcus litoralis (2015), FEBS Lett., 589, 3271-3276.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.147 E279D mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
2.7.1.147 E279L mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
2.7.1.147 E279Q mutation does not affect the KM value for MgADP-, but causes a large increase on KM value for glucose and an 87fold weaker binding of glucose onto the non-hydrolysable enzyme-AMP-AlF3 complex. Mutant is still inhibited by free Mg2+ Thermococcus litoralis
2.7.1.147 E308Q mutation increases the KM value for MgADP-, mutant is activated by free Mg2+ Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.147 0.014
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.034
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.066
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.27
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.28
-
 D-glucose wild-type, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.39
-
 D-glucose mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 1.46
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 8
-
 D-glucose mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 8.9
-
 D-glucose mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 109
-
 D-glucose mutant E279L, pH 7.8, 40°C Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.147 Thermococcus litoralis Q7M537
-
-
2.7.1.147 Thermococcus litoralis DSM 5473 Q7M537
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis AMP + D-glucose 6-phosphate
-
 ?
2.7.1.147 ADP + D-glucose
-
 Thermococcus litoralis DSM 5473 AMP + D-glucose 6-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.147 0.56
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.71
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 1.52
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 4.3
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 58
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.147 0.005
-
 D-glucose mutant E279L, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.079
-
 D-glucose mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 0.19
-
 D-glucose mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 2.95
-
 ADP mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 5.63
-
 ADP mutant E279D, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 10.7
-
 ADP mutant E279Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 11
-
 D-glucose mutant E308Q, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 16.4
-
 ADP mutant E279L, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 207
-
 D-glucose wild-type, pH 7.8, 40°C Thermococcus litoralis
2.7.1.147 4140
-
 ADP wild-type, pH 7.8, 40°C Thermococcus litoralis