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Literature summary extracted from

  • Yu, X.; Wang, X.; Engel, P.C.
    The specificity and kinetic mechanism of branched-chain amino acid aminotransferase from Escherichiacoli studied with a new improved coupled assay procedure and the enzymes potential for biocatalysis (2014), FEBS J., 281, 391-400.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.42 analysis development of a coupled assay, employing (R)-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans as an auxiliary enzyme, to provide accurate and reliable kinetic constants Escherichia coli
2.6.1.42 synthesis enzyme can be used for asymmetric synthesis of a range of non-natural amino acids such as L-norleucine, L-norvaline and L-neopentylglycine Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42
-
Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.42 molecular modelling of substrate binding. The inner side of the active site pocket binds the hydrophobic side chain and the alpha-carboxylate group of the substrates Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 0.07
-
3-methyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.08
-
2-oxoisocaproate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.08
-
4,4-dimethyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.2
-
2-oxoisovalerate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.22
-
2-Oxohexanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 0.6
-
2-oxovalerate pH 8.0, 37°C Escherichia coli
2.6.1.42 20.9
-
L-glutamate cosubstrate 4,4-dimethyl-2-oxopentanoate, pH 8.0, 37°C Escherichia coli
2.6.1.42 21.8
-
L-glutamate cosubstrate 2-oxoisocaproate, pH 8.0, 37°C Escherichia coli
2.6.1.42 26.5
-
L-glutamate cosubstrate 2-oxohexanoate, pH 8.0, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.42 35000
-
x * 35000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Escherichia coli P0AB80
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.42 L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate bibi ping-pong mechanism. For good 2-oxoacid substrates, release of 2-oxoglutarate is much slower than release of the product amino acid during the transamination reaction Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 2-oxohexanoate + L-glutamate
-
Escherichia coli L-norleucine + 2-oxoglutarate
-
?
2.6.1.42 2-oxoisocaproate + L-glutamate
-
Escherichia coli L-leucine + 2-oxoglutarate
-
?
2.6.1.42 2-oxoisovalerate + L-glutamate
-
Escherichia coli L-valine + 2-oxoglutarate
-
?
2.6.1.42 2-oxovalerate + L-glutamate
-
Escherichia coli L-norvaline + 2-oxoglutarate
-
?
2.6.1.42 3-methyl-2-oxopentanoate + L-glutamate
-
Escherichia coli L-isoleucine + 2-oxoglutarate
-
?
2.6.1.42 4,4-dimethyl-2-oxopentanoate + L-glutamate
-
Escherichia coli L-neopentylglycine + 2-oxoglutarate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.42 ? x * 35000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.42 IlvE
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.42 10.5
-
2-oxoisovalerate pH 8.0, 37°C Escherichia coli
2.6.1.42 12.4
-
4,4-dimethyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 23
-
3-methyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 23.1
-
2-Oxohexanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 24.7
-
2-oxoisocaproate pH 8.0, 37°C Escherichia coli
2.6.1.42 25.9
-
2-oxovalerate pH 8.0, 37°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.42 43.2
-
2-oxovalerate pH 8.0, 37°C Escherichia coli
2.6.1.42 52.5
-
2-oxoisovalerate pH 8.0, 37°C Escherichia coli
2.6.1.42 105
-
2-Oxohexanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 155
-
4,4-dimethyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli
2.6.1.42 309
-
2-oxoisocaproate pH 8.0, 37°C Escherichia coli
2.6.1.42 329
-
3-methyl-2-oxopentanoate pH 8.0, 37°C Escherichia coli