EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.8 | CuSO4 | 1 mM, 41% inhibition of xylanase 2, no inhibition of xylanase 1 | Humicola insolens | |
3.2.1.8 | HgCl2 | 1 mM, 85% inhibition of xylanase 2, 49% inhibition of xylanase 1 | Humicola insolens | |
3.2.1.8 | MnCl2 | 1 mM, 29% inhibition of xylanase 2, 20% inhibition of xylanase 1 | Humicola insolens | |
3.2.1.8 | NiCl2 | 1 mM, 19% inhibition of xylanase 2, no inhibition of xylanase 1 | Humicola insolens | |
3.2.1.8 | Pb(NO3)2 | 1 mM, 43% inhibition of xylanase 2, no inhibition of xylanase 1 | Humicola insolens | |
3.2.1.8 | ZnSO4 | 1 mM, 19% inhibition of xylanase 2, no inhibition of xylanase 1 | Humicola insolens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6000 | - |
xylanase 1, SDS-PAGE, mass spectrometry | Humicola insolens |
3.2.1.8 | 21000 | - |
xylanase 2, SDS-PAGE | Humicola insolens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Humicola insolens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.8 | glycoprotein | - |
Humicola insolens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | - |
Humicola insolens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | additional information | xylanase 1 and xylanase 2 are both inactive toward Avicel, carboxymethylcellulose, beta-glucan, arabinan (linear and branched), and (galacto)mannans. NO activity with xylotriose | Humicola insolens | ? | - |
? | |
3.2.1.8 | xylan + H2O | activity towards xylans from beech, birch, larch, and arabinoxylans from wheat is higher for xylanase 2 compared to xylanase 1 | Humicola insolens | ? | - |
? | |
3.2.1.8 | xyloheptaose + H2O | - |
Humicola insolens | ? | - |
? | |
3.2.1.8 | xylohexaose + H2O | - |
Humicola insolens | ? | - |
? | |
3.2.1.8 | xylopentaose + H2O | - |
Humicola insolens | ? | - |
? | |
3.2.1.8 | xylotetraose + H2O | low activity | Humicola insolens | 2 xylobiose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | ? | x * 21000, xylanase 2, SDS-PAGE | Humicola insolens |
3.2.1.8 | ? | x * 6000, xylanase 1, SDS-PAGE | Humicola insolens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | xylanase 1 | - |
Humicola insolens |
3.2.1.8 | xylanase 2 | - |
Humicola insolens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 55 | 60 | xylanase 1, xylanase 2 | Humicola insolens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 60 | - |
45 min, xylanase 2 completely loses activity, xylanase 1 retains 50% of its activity | Humicola insolens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6 | 6.5 | xylanase 1, xylanase 2 | Humicola insolens |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6 | 9 | 15 h, xylanase 2 is stable | Humicola insolens |
3.2.1.8 | 8 | - |
15 h, xylanase 1 loses 46% of its activity | Humicola insolens |
3.2.1.8 | 9 | - |
15 h, xylanase 1 loses 15% of its activity | Humicola insolens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.8 | Humicola insolens | xylanase 2, isoelectric focusing | - |
7.7 |
3.2.1.8 | Humicola insolens | xylanase 1, isoelectric focusing | - |
9 |