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Literature summary extracted from

  • Jorge, C.; Borges, N.; Santos, H.
    A novel pathway for the synthesis of inositol phospholipids uses cytidine diphosphate (CDP)-inositol as donor of the polar head group (2015), Environ. Microbiol., 17, 2492-2504.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.74 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate i.e. CHAPS, or Triton X-100, absolutely required Rhodothermus marinus
2.7.7.74 additional information presence of detergent is absolutely required Rhodothermus marinus
2.7.7.74 Triton X-100 or CHAPS, absolutely required Rhodothermus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.74 1.8
-
 CTP 75°C, pH 8.0 Rhodothermus marinus
2.7.7.74 2.6
-
 Inositol 1-phosphate 75°C, pH 8.0 Rhodothermus marinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.74 Co2+ 20 mM, 11% of the activity with Mg2+ Rhodothermus marinus
2.7.7.74 Mg2+ absolutely required, maximum activity at 20 mM Rhodothermus marinus
2.7.7.74 additional information no activity with Ni2+ Rhodothermus marinus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.74 Rhodothermus marinus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.74 59.8
-
 75°C, pH 8.0 Rhodothermus marinus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.74 CTP + inositol 1-phosphate
-
 Rhodothermus marinus diphosphate + CDP-inositol
-
 ?
2.7.7.74 additional information enzyme is highly specific for CTP and inositol-1-phosphate. No substrates: ATP, GTP, UTP, myo-inositol, glycerol, DL-glycerol-phosphate, alpha-glucose-1-phosphate, beta-glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1-phosphate and galactose-1-phosphate Rhodothermus marinus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.74 ICPT
-
 Rhodothermus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.74 95
-
-
 Rhodothermus marinus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.74 45
-
 no activity below Rhodothermus marinus
2.7.7.74 75
-
 about one third of the activity at 95°C Rhodothermus marinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.74 75
-
 half-life 5 h Rhodothermus marinus
2.7.7.74 85
-
 10 min, 80% residual activity Rhodothermus marinus
2.7.7.74 90
-
 10 min, 12% residual activity Rhodothermus marinus