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Literature summary extracted from

  • Baumlova, A.; Chalupska, D.; Rozycki, B.; Jovic, M.; Wisniewski, E.; Klima, M.; Dubankova, A.; Kloer, D.P.; Nencka, R.; Balla, T.; Boura, E.
    The crystal structure of the phosphatidylinositol 4-kinase IIalpha (2014), EMBO Rep., 15, 1085-1092.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.67 expressed in Escherichia coli BL21 Star cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.67 phosphatidylinositol 4-kinase type IIalpha in complex with ATP, vapor diffusion method Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.67 K165-K172 when the entire membrane binding segment K165-K172 was mutated to alanines, the kinase is inactive Homo sapiens
2.7.1.67 N163A the mutant shows about 10% of wild type activity Homo sapiens
2.7.1.67 N249A the mutant shows slightly reduced wild type activity Homo sapiens
2.7.1.67 R275A the mutant shows about 15% of wild type activity Homo sapiens
2.7.1.67 V339A the mutant shows slightly reduced wild type activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol Homo sapiens
-
 ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.67 Homo sapiens Q9BTU6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.67 affinity, anion exchange column chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.67 ATP + 1-phosphatidyl-1D-myo-inositol
-
 Homo sapiens ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.67 phosphatidylinositol 4-kinase type IIalpha
-
 Homo sapiens
2.7.1.67 PI4K IIalpha
-
 Homo sapiens