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Literature summary extracted from

  • Ueda, D.; Yamaga, H.; Murakami, M.; Totsuka, Y.; Shinada, T.; Sato, T.
    Biosynthesis of sesterterpenes, head-to-tail triterpenes, and sesquarterpenes in Bacillus clausii: identification of multifunctional enzymes and analysis of isoprenoid metabolites (2015), ChemBioChem, 16, 1371-1377.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.B41 gene bcl-hept, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme from pCold I vector as soluble protein in Escherichia coli strain BL21(DE3) Alkalihalobacillus clausii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.B41 Mg2+ required Alkalihalobacillus clausii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Alkalihalobacillus clausii heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate Alkalihalobacillus clausii JCM 9138 heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + 2 isopentenyl diphosphate Alkalihalobacillus clausii heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + 2 isopentenyl diphosphate Alkalihalobacillus clausii JCM 9138 heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + isopentenyl diphosphate Alkalihalobacillus clausii heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
2.5.1.B41 additional information Alkalihalobacillus clausii formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate ?
-
 ?
2.5.1.B41 additional information Alkalihalobacillus clausii JCM 9138 formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.B41 Alkalihalobacillus clausii S6AGL5 gene bcl-hept
-
2.5.1.B41 Alkalihalobacillus clausii S6B6P7 gene bcl-hept
-
2.5.1.B41 Alkalihalobacillus clausii JCM 9138 S6AGL5 gene bcl-hept
-
2.5.1.B41 Alkalihalobacillus clausii JCM 9138 S6B6P7 gene bcl-hept
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.B41 recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Alkalihalobacillus clausii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate (3) Alkalihalobacillus clausii
a
2.5.1.B41 geranylgeranyl diphosphate + 2 isopentenyl diphosphate = 2 diphosphate + all-trans-hexaprenyl diphosphate (2) Alkalihalobacillus clausii
a
2.5.1.B41 geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate (1) Alkalihalobacillus clausii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity Alkalihalobacillus clausii 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 Alkalihalobacillus clausii 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30, low activity Alkalihalobacillus clausii JCM 9138 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 (2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.30 Alkalihalobacillus clausii JCM 9138 4 diphosphate + all-trans-heptaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + 2 isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 Alkalihalobacillus clausii 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + 2 isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.82 Alkalihalobacillus clausii JCM 9138 2 diphosphate + all-trans-hexaprenyl diphosphate
-
 ?
2.5.1.B41 geranylgeranyl diphosphate + isopentenyl diphosphate heterodimeric E-IDS (HepS/T), cf. EC 2.5.1.81 Alkalihalobacillus clausii (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate
-
 ?
2.5.1.B41 additional information formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate Alkalihalobacillus clausii ?
-
 ?
2.5.1.B41 additional information product identification and determination by mass spectrometry, NMR spectrometry, and TLC Alkalihalobacillus clausii ?
-
 ?
2.5.1.B41 additional information formation of geranylfarnesyl diphosphate and hexaprenyl diphosphate is mediated by heteromeric E-IDS2-S/L. E-IDS2-S or E-IDS2-L alone form no product from dimethylallyl diphosphate, (2E,6E)-farnesyl diphosphate, and geranylgeranyl diphosphate Alkalihalobacillus clausii JCM 9138 ?
-
 ?
2.5.1.B41 additional information product identification and determination by mass spectrometry, NMR spectrometry, and TLC Alkalihalobacillus clausii JCM 9138 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.B41 (all-E)-isoprenyl diphosphate synthase
-
 Alkalihalobacillus clausii
2.5.1.B41 E-IDS2-L
-
 Alkalihalobacillus clausii
2.5.1.B41 E-IDS2-S
-
 Alkalihalobacillus clausii
2.5.1.B41 geranylfarnesyl diphosphate/hexaprenyl diphosphate/heptaprenyl diphosphate synthase
-
 Alkalihalobacillus clausii
2.5.1.B41 GFPP/HexPP/HepPP synthase
-
 Alkalihalobacillus clausii
2.5.1.B41 HepS
-
 Alkalihalobacillus clausii
2.5.1.B41 HepT
-
 Alkalihalobacillus clausii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.B41 37
-
 assay at Alkalihalobacillus clausii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.B41 8.5
-
 assay at Alkalihalobacillus clausii

General Information

EC Number General Information Comment Organism
2.5.1.B41 metabolism hexaprenyl diphosphate and heptaprenyl diphosphate, intermediates of two isoprenoid pathways (acyclic terpenes and menaquinones), are biosynthesized by one trifunctional (all-E)-isoprenyl diphosphate synthase, E-IDS. Geranylfarnesyl diphosphate/hexaprenyl diphosphate and heptaprenyl diphosphate are the primary substrates for the biosynthesis of acyclic terpenes and menaquinone-7, respectively. (All-E)-and E,Z-mixed isoprenyl diphosphates are formed by E-IDSs and Z-IDSs, respectively, which have no sequence similarity with each other Alkalihalobacillus clausii
2.5.1.B41 physiological function all-trans-heptaprenyl diphosphate is biosynthesized from isopentenyl diphosphate and farnesyl diphosphate by heterodimeric E-IDS (HepS/T), and is used to produce menaquinone-7 Alkalihalobacillus clausii