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Literature summary extracted from

  • Kusumanchi, P.; Zhang, Y.; Jani, M.B.; Jayaram, N.H.; Khan, R.A.; Tang, Y.; Antony, A.C.; Jayaram, H.N.
    Nicotinamide mononucleotide adenylyltransferase2 overexpression enhances colorectal cancer cell-kill by Tiazofurin (2013), Cancer Gene Ther., 20, 403-412.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.1 gene NMNAT2, transfection of hNMNAT2 results in a six- and threefold cytoplasmic overexpression in Caco-2 and HT-29 cell lines, respectively, correlating with Tiazofurin-induced enhanced cell killing Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.1 cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide Homo sapiens
-
 diphosphate + NAD+
-
 r
2.7.7.1 ATP + tiazofurin Homo sapiens tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 diphosphate + thiazole-4-carboxamide adenine dinucleotide
-
 ?
2.7.7.1 additional information Homo sapiens bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.1 Homo sapiens Q9BZQ4 gene NMNAT2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.1 Caco-2 cell
-
 Homo sapiens
-
2.7.7.1 colorectal cancer cell exhibits low levels of NMNAT2 Homo sapiens
-
2.7.7.1 HT-29 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.1 ATP + nicotinamide ribonucleotide
-
 Homo sapiens diphosphate + NAD+
-
 r
2.7.7.1 ATP + tiazofurin tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 Homo sapiens diphosphate + thiazole-4-carboxamide adenine dinucleotide
-
 ?
2.7.7.1 additional information bifunctional enzyme, that also shows nicotinate-nucleotide adenylyltransferase activity, EC 2.7.7.18 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.1 nicotinamide mononucleotide adenylyltransferase2
-
 Homo sapiens
2.7.7.1 NMNAT2
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.7.1 malfunction colorectal cancer cells, that exhibit low levels of NMNAT2 and are refractory to tiazofurin, can be rendered sensitive to Tiazofurin by overexpressing NMNAT2 Homo sapiens
2.7.7.1 physiological function tiazofurin is a pro-drug that is metabolized by cytosolic nicotinamide mononucleotide adenylyltransferase2 (NMNAT2) to thiazole-4-carboxamide adenine dinucleotide, a potent inhibitor of inosine 5'-monophosphate dehydrogenase required for cellular guanylate synthesis. Resistance of colorectal cancer cell-kill to Tiazofurin can be overcome by sequentially overexpressing hNMNAT2 and then facilitating the uptake of Tiazofurin by folate-tethered nanoparticles, which enter cells via folate receptors Homo sapiens