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Literature summary extracted from

  • Kato, S.; Takaichi, S.; Ishikawa, T.; Asahina, M.; Takahashi, S.; Shinomura, T.
    Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoene synthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis (2016), BMC Plant Biol., 16, 4-15.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.32 gene crtB, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.32 additional information no predictable plastid transit peptide in the N-terminal region of EgCrtB. The stigma of the alga is surrounded by a single membrane and has no structural similarity to the chloroplast. This suggests that EgCrtB might be transported to stigmata as well as plastids and that EgCrtB might contain an as-yet unidentified signal sequence Euglena gracilis
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Organism

EC Number Organism UniProt Comment Textmining
2.5.1.32 Euglena gracilis A0A0U5ARE7 gene crtB
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2.5.1.32 Euglena gracilis Z A0A0U5ARE7 gene crtB
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Synonyms

EC Number Synonyms Comment Organism
2.5.1.32 EgcrtB
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 Euglena gracilis
2.5.1.32 phytoene synthase
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 Euglena gracilis

Expression

EC Number Organism Comment Expression
2.5.1.32 Euglena gracilis the expression of gene crtB in cells cultured at 25°C under continuous illumination increases 1.3fold relative to control up

General Information

EC Number General Information Comment Organism
2.5.1.32 evolution Euglena gracilis CrtB belongs to a clade that is distinct from groups of algae and higher plants Euglena gracilis
2.5.1.32 metabolism the enzyme catalyzes an early step of carotenoid biosynthesis. The carotenoid biosynthetic pathway responds to excess light stress at the level of crtB transcription Euglena gracilis