EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.1 | synthesis | under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h | Kangiella koreensis |
1.1.1.105 | synthesis | under optimized conditions, the enzyme produces 600 mg all-trans-retinol per l after 3 h, with a conversion yield of 27.3% (w/w) and a productivity of 200 mg per l and h | Kangiella koreensis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.1 | expression in Escherichia coli | Kangiella koreensis |
1.1.1.105 | expressed in Escherichia coli ER2566 cells | Kangiella koreensis |
1.1.1.105 | expression in Escherichia coli | Kangiella koreensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.105 | additional information | the enzyme is not influenced by EDTA | Kangiella koreensis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.105 | 0.0018 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.105 | additional information | the enzyme activity is not affected by monovalent or divalent cations | Kangiella koreensis | |
1.1.1.105 | Zn2+ | contains Zn2+ | Kangiella koreensis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 39900 | - |
- |
Kangiella koreensis |
1.1.1.1 | 40000 | - |
- |
Kangiella koreensis |
1.1.1.105 | 39900 | - |
- |
Kangiella koreensis |
1.1.1.105 | 40000 | - |
- |
Kangiella koreensis |
1.1.1.105 | 40000 | - |
2 * 40000, SDS-PAGE | Kangiella koreensis |
1.1.1.105 | 80000 | - |
gel filtration | Kangiella koreensis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.105 | all-trans-retinal + NADH + H+ | Kangiella koreensis | - |
all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinal + NADH + H+ | Kangiella koreensis SW-125 | - |
all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinol + NAD+ | Kangiella koreensis | - |
all-trans-retinal + NADH + H+ | - |
r | |
1.1.1.105 | all-trans-retinol + NAD+ | Kangiella koreensis SW-125 | - |
all-trans-retinal + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.1 | Kangiella koreensis | C7R702 | - |
- |
1.1.1.105 | Kangiella koreensis | - |
- |
- |
1.1.1.105 | Kangiella koreensis | C7R702 | - |
- |
1.1.1.105 | Kangiella koreensis SW-125 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.105 | immobilized metal ion affinity chromatography | Kangiella koreensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.1 | 79.6 | - |
substrate butan-1-ol, pH 6.5, 60°C | Kangiella koreensis |
1.1.1.105 | 0.0213 | - |
pH 6.5, 60°C | Kangiella koreensis |
1.1.1.105 | 0.0213 | - |
with all-trans-retinal as substrate, at pH 6.5 and 60°C | Kangiella koreensis |
1.1.1.105 | 0.024 | - |
with all-trans-retinol as substrate, at pH 6.5 and 60°C | Kangiella koreensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.1 | acetaldehyde + NADH + H+ | 83.2% of the activity with butan-1-ol | Kangiella koreensis | ethanol + NAD+ | - |
? | |
1.1.1.1 | all-trans retinal + NADH + H+ | 26.8% of the activity with butan-1-ol | Kangiella koreensis | all-trans retinol + NAD+ | - |
? | |
1.1.1.1 | butan-1-ol + NAD+ | - |
Kangiella koreensis | butanal + NADH + H+ | - |
? | |
1.1.1.1 | hexan-1-ol + NAD+ | 77.1% of the activity with butan-1-ol | Kangiella koreensis | hexanal + NADH + H+ | - |
? | |
1.1.1.1 | additional information | enzyme is an alcohol dehydrogenase with additional activity for all-trans-retinol, reaction of EC 1.1.1.184 | Kangiella koreensis | ? | - |
? | |
1.1.1.1 | propan-1-ol + NAD+ | 64.3% of the activity with butan-1-ol | Kangiella koreensis | propanal + NADH + H+ | - |
? | |
1.1.1.105 | all-trans retinal + NADH + H+ | 26.8% of the activity with butan-1-ol | Kangiella koreensis | all-trans retinol + NAD+ | - |
? | |
1.1.1.105 | all-trans-retinal + NADH + H+ | - |
Kangiella koreensis | all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinal + NADH + H+ | 26% activity compared to butyl alcohol | Kangiella koreensis | all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinal + NADH + H+ | - |
Kangiella koreensis SW-125 | all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinal + NADH + H+ | 26% activity compared to butyl alcohol | Kangiella koreensis SW-125 | all-trans-retinol + NAD+ | - |
r | |
1.1.1.105 | all-trans-retinol + NAD+ | - |
Kangiella koreensis | all-trans-retinal + NADH + H+ | - |
r | |
1.1.1.105 | all-trans-retinol + NAD+ | 30.2% activity compared to butyl alcohol | Kangiella koreensis | all-trans-retinal + NADH + H+ | - |
? | |
1.1.1.105 | all-trans-retinol + NAD+ | - |
Kangiella koreensis SW-125 | all-trans-retinal + NADH + H+ | - |
r | |
1.1.1.105 | all-trans-retinol + NAD+ | 30.2% activity compared to butyl alcohol | Kangiella koreensis SW-125 | all-trans-retinal + NADH + H+ | - |
? | |
1.1.1.105 | butyl alcohol + NAD+ | 100% activity | Kangiella koreensis | butanal + NADH + H+ | - |
? | |
1.1.1.105 | butyl alcohol + NAD+ | 100% activity | Kangiella koreensis SW-125 | butanal + NADH + H+ | - |
? | |
1.1.1.105 | additional information | enzyme is an alcohol dehydrogenase, EC 1.1.1.1, with activity for all-trans-retinol using NAD+ as a cofactor | Kangiella koreensis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.1 | ? | x * 40000, SDS-PAGE, x * 39900, calculated | Kangiella koreensis |
1.1.1.105 | ? | x * 40000, SDS-PAGE, x * 39900, calculated | Kangiella koreensis |
1.1.1.105 | homodimer | 2 * 40000, SDS-PAGE | Kangiella koreensis |
1.1.1.105 | homodimer | 2 * 39900, cslculated from amino acid sequence | Kangiella koreensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.105 | ADH | - |
Kangiella koreensis |
1.1.1.105 | alcohol dehydrogenase | - |
Kangiella koreensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.105 | 60 | - |
- |
Kangiella koreensis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.105 | 0.028 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.105 | 6.5 | - |
- |
Kangiella koreensis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.1 | NAD+ | - |
Kangiella koreensis | |
1.1.1.1 | NADH | - |
Kangiella koreensis | |
1.1.1.105 | NAD+ | - |
Kangiella koreensis | |
1.1.1.105 | NADH | - |
Kangiella koreensis | |
1.1.1.105 | NADP+ | - |
Kangiella koreensis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.105 | 15.7 | - |
all-trans-retinal | at pH 6.5 and 60°C | Kangiella koreensis |