EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.30 | expressed in Escherichia coli BL21(DE3) cells | Alcaligenes faecalis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.30 | H144L | the mutant shows activity with levulinic acid | Alcaligenes faecalis |
1.1.1.30 | H144L/W187F | the mutant shows activity with levulinic acid | Alcaligenes faecalis |
1.1.1.30 | W187F | the mutant shows activity with levulinic acid | Alcaligenes faecalis |
EC Number | General Stability | Organism |
---|---|---|
1.1.1.30 | counterparts | Alcaligenes faecalis |
1.1.1.30 | the enzyme with an N-terminal His-tag has approximately 1200fold higher catalytic efficiency than its C-terminal counterpart and exhibits higher catalytic efficiency for levulinic acid than the C-terminal | Alcaligenes faecalis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.30 | 0.17 | - |
acetoacetate | recombinant enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.63 | - |
levulinic acid | recombinant mutant enzyme W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.64 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.78 | - |
levulinic acid | recombinant wild type enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.91 | - |
levulinic acid | recombinant mutant enzyme H144L with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 8.25 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 10.47 | - |
acetoacetate | recombinant enzyme with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 16.98 | - |
levulinic acid | recombinant mutant enzyme H144L with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.30 | acetoacetate + NADH + H+ | Alcaligenes faecalis | - |
(R)-3-hydroxybutanoate + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.30 | Alcaligenes faecalis | D0VWQ0 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.30 | acetoacetate + NADH + H+ | - |
Alcaligenes faecalis | (R)-3-hydroxybutanoate + NAD+ | - |
? | |
1.1.1.30 | levulinic acid + NADH + H+ | only the recombinant wild type enzyme with N-terminal His-tag shows activity with levulinic acid | Alcaligenes faecalis | 4-hydroxyvaleric acid + NAD+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.30 | 3HBDH | - |
Alcaligenes faecalis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.30 | 0.006 | - |
levulinic acid | recombinant wild type enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.019 | - |
levulinic acid | recombinant mutant enzyme W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.028 | - |
levulinic acid | recombinant mutant enzyme H144L with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.06 | - |
levulinic acid | recombinant mutant enzyme H144L with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.079 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 0.164 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 4.9 | - |
acetoacetate | recombinant enzyme with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 97.16 | - |
acetoacetate | recombinant enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.30 | NADH | - |
Alcaligenes faecalis |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.30 | 0.47 | - |
acetoacetate | recombinant enzyme with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 1.62 | - |
levulinic acid | recombinant mutant enzyme H144L with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 7.68 | - |
levulinic acid | recombinant wild type enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 9.63 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with C-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 29.69 | - |
levulinic acid | recombinant mutant enzyme W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 65.94 | - |
levulinic acid | recombinant mutant enzyme H144L with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 256.5 | - |
levulinic acid | recombinant mutant enzyme H144L/W187F with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis | |
1.1.1.30 | 560.1 | - |
acetoacetate | recombinant enzyme with N-terminal His-tag, at pH 6.5 and 30°C | Alcaligenes faecalis |