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Literature summary extracted from

  • Kelle, S.; Nieter, A.; Krings, U.; Zelena, K.; Linke, D.; Berger, R.
    Heterologous production of a feruloyl esterase from Pleurotus sapidus synthesizing feruloyl-saccharide esters (2015), Biotechnol. Appl. Biochem., 63, 852-862.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Escherichia coli and Pichia pastoris Pleurotus sapidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.227
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 0.427
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.73 extracellular
-
 Pleurotus sapidus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Pleurotus sapidus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.73 1397
-
 substrate 5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 1681
-
 substrate beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose, pH 5.0, 37°C Pleurotus sapidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-trans-feruloyl-L-arabinofuranose + H2O 83% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + L-arabinofuranose
-
 ?
3.1.1.73 alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose 48% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
3.1.1.73 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Pleurotus sapidus ferulic acid + beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
3.1.1.73 ferulic acid ethyl ester + H2O 62% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + ethanol
-
 ?
3.1.1.73 ferulic acid methyl ester + H2O 61% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus ferulic acid + methanol
-
 ?
3.1.1.73 additional information enzyme additionally catalyzes transferulation using methyl ferulate as a ferulic acid donor and glucose, fructose, or galactose as acceptor, leading to two ferulated monosaccharide anomers each Pleurotus sapidus ?
-
 ?
3.1.1.73 sinapinic acid methyl ester + H2O 22% of the activity with beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose Pleurotus sapidus sinapinic acid + methanol
-
 ?
3.1.1.73 wheat bran + H2O
-
 Pleurotus sapidus ferulic acid + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 Est1
-
 Pleurotus sapidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.73 50
-
-
 Pleurotus sapidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.73 20 40 stable for 1 h Pleurotus sapidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 1.2
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 1.96
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.73 5
-
-
 Pleurotus sapidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.73 3 8 no activity below or above Pleurotus sapidus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 2.81
-
 ferulic acid methyl ester pH 5.0, 37°C Pleurotus sapidus
3.1.1.73 8.64
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 5.0, 37°C Pleurotus sapidus