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Literature summary extracted from

  • Tani, Y.; Kimura, K.; Mihara, H.
    Purification and properties of 4-methyl-5-hydroxyethylthiazole kinase from Escherichia coli (2016), Biosci. Biotechnol. Biochem., 80, 514-517.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.50 Co2+ highest activity in the presence of Co2+ Escherichia coli
2.7.1.50 Mg2+ activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.50 28000
-
 x * 28000, SDS-PAGE Escherichia coli
2.7.1.50 28100
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.50 Escherichia coli P76423
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.50 ammonium sulfate precipitation, Ni-NTA column chromatography, and gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.50 0.9
-
 with UTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 1.1
-
 with CTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 1.4
-
 with ATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 3.2
-
 with GTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 3.9
-
 with dCTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 4
-
 with dUTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 4.3
-
 with UTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 5.2
-
 with CTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 8.9
-
 with TTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 9.2
-
 with dATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 11
-
 with ATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 11
-
 with dGTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 14
-
 with dGTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 14
-
 with dUTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 15
-
 with dCTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 24
-
 with GTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 37
-
 with dATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli
2.7.1.50 45
-
 with TTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.50 ATP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 CTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 dATP + 4-methyl-5-(2-hydroxyethyl)thiazole the enzyme shows a preference for dATP as a phosphate donor over ATP Escherichia coli dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 dCTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dCDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 dGTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dGDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 dUTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli dUDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 GTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 TTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli TDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?
2.7.1.50 UTP + 4-methyl-5-(2-hydroxyethyl)thiazole
-
 Escherichia coli UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.50 ? x * 28000, SDS-PAGE Escherichia coli
2.7.1.50 ? x * 28100, calculated from amino acid sequence Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.1.50 4-methyl-5-hydroxyethylthiazole kinase
-
 Escherichia coli
2.7.1.50 ThiM
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.50 30 60 no decrease in the enzyme activity is detected at temperatures lower than 50°C, although about 60% of its original activity is lost at 60°C after 20 min Escherichia coli