EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.27 | 3-phosphoglycerate | activation. A0.5 value and Hill coefficient for wild-type 0.076 mM and 1.0, large subunit mutant E38K 0.013 mM and 0.9, large subunit mutant G101N 0.023 mM and 1.0, large subunit mutant E38K/G101N 0.006 mM and 0.4 | Solanum tuberosum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.27 | E38K | the enzyme activities of large subunit mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type | Solanum tuberosum |
2.7.7.27 | E38K/G101N | in an Escherichia coli mutant defective in the synthesis of ADP-glucose, expression of large subunit mutant E38K/G101N mediates higher glycogen production than wild-type potato AGPase and the single mutant enzymes, E38K and G101N, individually. Purified large subunit mutant E38K/G101N shows higher sensitivity to 3-phosphoglycerate activation and tolerance to phosphate inhibition than mutants E38K or G101N. The enzyme activities of mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type | Solanum tuberosum |
2.7.7.27 | G101N | the enzyme activities of large subunit mutants E38K, G101N, and E38K/G101N are more readily stimulated by phosphate-ester metabolites, such as fructose 6-phosphate, fructose 2,6-bisphosphate, and ribose 5-phosphate, than that of wild-type | Solanum tuberosum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.27 | phosphate | - |
Solanum tuberosum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 0.074 | - |
alpha-D-glucose 1-phosphate | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.081 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.11 | - |
ATP | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.14 | - |
ATP | wild-type, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.15 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.16 | - |
ATP | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.17 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 0.17 | - |
ATP | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.27 | Solanum tuberosum | Q00081 | large subunit | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.7.27 | tuber | - |
Solanum tuberosum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.27 | ATP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + ADP-glucose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.27 | AGPS1 | - |
Solanum tuberosum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 101 | - |
alpha-D-glucose 1-phosphate | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 105 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 117 | - |
ATP | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 140 | - |
ATP | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 142 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 148 | - |
ATP | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 204 | - |
ATP | wild-type, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 266 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 7.5, 37°C | Solanum tuberosum |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 0.9 | - |
wild-type, pH 7.5, 37°C | Solanum tuberosum | phosphate | |
2.7.7.27 | 2.6 | - |
large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | phosphate | |
2.7.7.27 | 3.7 | - |
large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | phosphate | |
2.7.7.27 | 8.9 | - |
large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | phosphate |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.27 | 819 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 876 | - |
ATP | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 893 | - |
ATP | large subunit mutant E38K, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 1100 | - |
ATP | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 1300 | - |
alpha-D-glucose 1-phosphate | large subunit mutant E38K/G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 1360 | - |
alpha-D-glucose 1-phosphate | large subunit mutant G101N, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 1460 | - |
ATP | wild-type, pH 7.5, 37°C | Solanum tuberosum | |
2.7.7.27 | 1800 | - |
alpha-D-glucose 1-phosphate | wild-type, pH 7.5, 37°C | Solanum tuberosum |